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Assay Detail

CHEMBL2406002

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Binding
Binding affinity to GST-tagged MDM2 (unknown origin) assessed as inhibition of interaction with p53 after 1 hr by HTRF assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

E3 ubiquitin-protein ligase Mdm2 (CHEMBL5023)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of RG7388, a potent and selective p53-MDM2 inhibitor in clinical development.
Ding Q, Zhang Z, Liu JJ, Jiang N, Zhang J, Ross TM, Chu XJ, Bartkovitz D, Podlaski F, Janson C, Tovar C, Filipovic ZM, Higgins B, Glenn K, Packman K, Vassilev LT, Graves B.
J Med Chem (2013) 56 : 5979 -5983
PubMed 23808545 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.53 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2402737 IDASANUTLIN 3.0 1 8.22
CHEMBL2386346 RO-5045337 1.0 1 7.75
CHEMBL2402734 1 7.70
CHEMBL2402735 1 7.68
CHEMBL2402733 1 7.66
CHEMBL2402731 1 7.66
CHEMBL2402732 1 7.64
CHEMBL2402736 1 7.60
CHEMBL2402730 1 7.38
CHEMBL2402729 1 7.25
CHEMBL2402574 1 7.13
CHEMBL2402573 1 6.71

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2402737 IDASANUTLIN IC50 = 6.0 nM 8.22
CHEMBL2386346 RO-5045337 IC50 = 18.0 nM 7.75
CHEMBL2402734 IC50 = 20.0 nM 7.70
CHEMBL2402735 IC50 = 21.0 nM 7.68
CHEMBL2402733 IC50 = 22.0 nM 7.66
CHEMBL2402731 IC50 = 22.0 nM 7.66
CHEMBL2402732 IC50 = 23.0 nM 7.64
CHEMBL2402736 IC50 = 25.0 nM 7.60
CHEMBL2402730 IC50 = 42.0 nM 7.38
CHEMBL2402729 IC50 = 56.0 nM 7.25
CHEMBL2402574 IC50 = 74.0 nM 7.13
CHEMBL2402573 IC50 = 196.0 nM 6.71