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Assay Detail

CHEMBL2406149

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of acetylcholinesterase (unknown origin) using acetylcholine iodide as substrate preincubated for 15 mins prior to substrate addition by spectrophotometric analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New bioactive dihydrofuranocoumarins from the roots of the Tunisian Ferula lutea (Poir.) Maire.
Ben Salem S, Jabrane A, Harzallah-Skhiri F, Ben Jannet H.
Bioorg Med Chem Lett (2013) 23 : 4248 -4252
PubMed 23746477 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.62 8.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL94 PHYSOSTIGMINE 3.0 1 8.54
CHEMBL416 METHOXSALEN 4.0 1 6.12
CHEMBL164660 PSORALEN 1.0 1 5.19
CHEMBL145 CAFFEIC ACID 3.0 1 -
CHEMBL51628 UMBELLIFERONE 1 -
CHEMBL1443379 1 -
CHEMBL2402007 (-)-PRANTSCHIMGIN 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL94 PHYSOSTIGMINE IC50 = 2.9 nM 8.54
CHEMBL416 METHOXSALEN IC50 = 760.0 nM 6.12
CHEMBL164660 PSORALEN IC50 = 6480.0 nM 5.19
CHEMBL145 CAFFEIC ACID IC50 > 100000.0 nM -
CHEMBL51628 UMBELLIFERONE IC50 > 100000.0 nM -
CHEMBL1443379 IC50 > 100000.0 nM -
CHEMBL2402007 (-)-PRANTSCHIMGIN IC50 > 100000.0 nM -