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Assay Detail

CHEMBL2406229

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABL T315I mutant (unknown origin)-mediated phosphorylation of biotinylated poly-Glu-Tyr preincubated for 30 to 60 mins prior to substrate addition by TR-FRET assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase ABL1 (CHEMBL1862)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Targeting gain of function and resistance mutations in Abl and KIT by hybrid compound design.
Richters A, Ketzer J, Getlik M, Grütter C, Schneider R, Heuckmann JM, Heynck S, Sos ML, Gupta A, Unger A, Schultz-Fademrecht C, Thomas RK, Bauer S, Rauh D.
J Med Chem (2013) 56 : 5757 -5772
PubMed 23773153 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.62 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1171837 PONATINIB 4.0 1 9.00
CHEMBL2403815 1 7.09
CHEMBL2403816 1 6.92
CHEMBL510516 1 6.85
CHEMBL2403820 1 6.75
CHEMBL522892 DOVITINIB 3.0 1 6.64
CHEMBL2403814 1 6.45
CHEMBL2403818 1 6.36
CHEMBL535 SUNITINIB 4.0 1 6.30
CHEMBL2403813 1 6.25
CHEMBL2403821 1 6.09
CHEMBL2403819 1 5.93
CHEMBL2403817 1 5.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1171837 PONATINIB IC50 = 1.0 nM 9.00
CHEMBL2403815 IC50 = 81.0 nM 7.09
CHEMBL2403816 IC50 = 120.0 nM 6.92
CHEMBL510516 IC50 = 141.0 nM 6.85
CHEMBL2403820 IC50 = 176.0 nM 6.75
CHEMBL522892 DOVITINIB IC50 = 228.0 nM 6.64
CHEMBL2403814 IC50 = 355.0 nM 6.45
CHEMBL2403818 IC50 = 441.0 nM 6.36
CHEMBL535 SUNITINIB IC50 = 504.0 nM 6.30
CHEMBL2403813 IC50 = 561.0 nM 6.25
CHEMBL2403821 IC50 = 820.0 nM 6.09
CHEMBL2403819 IC50 = 1170.0 nM 5.93
CHEMBL2403817 IC50 = 3620.0 nM 5.44