Assay Detail
Binding
CHEMBL2412002
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Ptch mRNA expression after 24 hrs by RT-PCR analysis
8
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | C3H 10T1/2 |
| Confidence | 3 — Cell-line / Tissue |
| Curated By | Autocuration |
Publication
Identification of vitamin d3-based hedgehog pathway inhibitors that incorporate an aromatic a-ring isostere.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 5.60 | 5.96 |
| Inhibition | 2 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2407295 | — | — | 2 | 5.96 |
| CHEMBL2063141 | — | — | 1 | 5.55 |
| CHEMBL1042 | CHOLECALCIFEROL | 4.0 | 1 | 5.54 |
| CHEMBL2407297 | — | — | 2 | 5.35 |
| CHEMBL254129 | CYCLOPAMINE | — | 1 | - |
| CHEMBL473417 | VISMODEGIB | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2407295 | — | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL2063141 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL1042 | CHOLECALCIFEROL | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL2407297 | — | IC50 | = | 4500.0 | nM | 5.35 |
| CHEMBL2407297 | — | Inhibition | - | % | - | |
| CHEMBL2407295 | — | Inhibition | - | % | - | |
| CHEMBL254129 | CYCLOPAMINE | IC50 | - | — | - | |
| CHEMBL473417 | VISMODEGIB | IC50 | - | — | - |