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Assay Detail

CHEMBL2415373

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human 17beta-HSD5 expressed in HEK293 cells using androstenedione as substrate assessed as testosterone synthesis after 4 hrs
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldo-keto reductase family 1 member C3 (CHEMBL4681)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2-methyl-1-{1-[(5-methyl-1H-indol-2-yl)carbonyl]piperidin-4-yl}propan-2-ol: a novel, potent and selective type 5 17β-hydroxysteroid dehydrogenase inhibitor.
Watanabe K, Kakefuda A, Yasuda M, Enjo K, Kikuchi A, Furutani T, Naritomi Y, Otsuka Y, Okada M, Ohta M.
Bioorg Med Chem (2013) 21 : 5261 -5270
PubMed 23845281 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.05 9.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2413849 1 9.47
CHEMBL2413850 1 8.68
CHEMBL2413860 1 8.35
CHEMBL2413851 1 7.92
CHEMBL2413852 1 7.89
CHEMBL2413853 1 7.48
CHEMBL2413863 1 7.43
CHEMBL2413855 1 6.85
CHEMBL2413861 1 6.80
CHEMBL2413857 1 6.64
CHEMBL2413859 1 6.11
CHEMBL2413864 1 6.10
CHEMBL2413856 1 5.96
CHEMBL2413854 1 5.92
CHEMBL2413858 1 5.68
CHEMBL2413862 1 5.55
CHEMBL2413848 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2413849 IC50 = 0.34 nM 9.47
CHEMBL2413850 IC50 = 2.1 nM 8.68
CHEMBL2413860 IC50 = 4.5 nM 8.35
CHEMBL2413851 IC50 = 12.0 nM 7.92
CHEMBL2413852 IC50 = 13.0 nM 7.89
CHEMBL2413853 IC50 = 33.0 nM 7.48
CHEMBL2413863 IC50 = 37.0 nM 7.43
CHEMBL2413855 IC50 = 140.0 nM 6.85
CHEMBL2413861 IC50 = 160.0 nM 6.80
CHEMBL2413857 IC50 = 230.0 nM 6.64
CHEMBL2413859 IC50 = 770.0 nM 6.11
CHEMBL2413864 IC50 = 790.0 nM 6.10
CHEMBL2413856 IC50 = 1100.0 nM 5.96
CHEMBL2413854 IC50 = 1200.0 nM 5.92
CHEMBL2413858 IC50 = 2100.0 nM 5.68
CHEMBL2413862 IC50 = 2800.0 nM 5.55
CHEMBL2413848 IC50 > 10000.0 nM -