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Assay Detail

CHEMBL2417028

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GAT3 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 3 (CHEMBL5208)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cyclopropane-based conformational restriction of GABA by a stereochemical diversity-oriented strategy: identification of an efficient lead for potent inhibitors of GABA transports.
Nakada K, Yoshikawa M, Ide S, Suemasa A, Kawamura S, Kobayashi T, Masuda E, Ito Y, Hayakawa W, Katayama T, Yamada S, Arisawa M, Minami M, Shuto S.
Bioorg Med Chem (2013) 21 : 4938 -4950
PubMed 23886812 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.87 5.95

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL75035 1 5.95
CHEMBL2415009 1 4.86
CHEMBL297569 BETA-ALANINE 1 4.38
CHEMBL77575 (R)-NIPECOTIC ACID 1 4.29
CHEMBL77484 (S)-NIPECOTIC ACID 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL75035 IC50 = 1120.0 nM 5.95
CHEMBL2415009 IC50 = 13900.0 nM 4.86
CHEMBL297569 BETA-ALANINE IC50 = 41200.0 nM 4.38
CHEMBL77575 (R)-NIPECOTIC ACID IC50 = 51000.0 nM 4.29
CHEMBL77484 (S)-NIPECOTIC ACID IC50 = 2320000.0 nM -