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Assay Detail

CHEMBL2417341

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Reversible inhibition of human recombinant FAAH using N-arachidonyl-7-amino-4-methylcoumarin as substrate preincubated for 20 mins before substrate addition by fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL2243)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Macamides and their synthetic analogs: evaluation of in vitro FAAH inhibition.
Wu H, Kelley CJ, Pino-Figueroa A, Vu HD, Maher TJ.
Bioorg Med Chem (2013) 21 : 5188 -5197
PubMed 23891163 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.81 7.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL177577 1 7.48
CHEMBL2413160 1 4.99
CHEMBL2415105 1 4.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL177577 IC50 = 33.0 nM 7.48
CHEMBL2413160 IC50 = 10300.0 nM 4.99
CHEMBL2415105 IC50 = 11000.0 nM 4.96