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Assay Detail

CHEMBL2421059

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Sprague Dawley rat lung native NAAA enzyme using heptadecenoylethanolamide as substrate preincubated for 30 mins followed by substrate addition measured after 30 mins by UPLC-MS analysis
30
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

N-acylethanolamine-hydrolyzing acid amidase (CHEMBL2034805)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and structure-activity relationship (SAR) of 2-methyl-4-oxo-3-oxetanylcarbamic acid esters, a class of potent N-acylethanolamine acid amidase (NAAA) inhibitors.
Ponzano S, Bertozzi F, Mengatto L, Dionisi M, Armirotti A, Romeo E, Berteotti A, Fiorelli C, Tarozzo G, Reggiani A, Duranti A, Tarzia G, Mor M, Cavalli A, Piomelli D, Bandiera T.
J Med Chem (2013) 56 : 6917 -6934
PubMed 23991897 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.72 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2419814 1 8.15
CHEMBL2419830 1 7.89
CHEMBL2419811 1 7.80
CHEMBL2419808 3 7.75
CHEMBL2419819 1 7.70
CHEMBL2419827 1 7.52
CHEMBL2419826 1 7.40
CHEMBL2064166 1 7.30
CHEMBL2419828 1 7.30
CHEMBL2419810 1 7.22
CHEMBL2419807 3 7.10
CHEMBL2419821 1 7.05
CHEMBL2419829 1 6.85
CHEMBL2419825 1 6.57
CHEMBL2419812 1 6.57
CHEMBL2417575 1 6.54
CHEMBL2419809 1 6.51
CHEMBL2419815 1 6.51
CHEMBL2419822 1 6.41
CHEMBL2419818 1 6.32
CHEMBL2419824 1 6.12
CHEMBL2419823 1 5.93
CHEMBL2419820 1 5.60
CHEMBL2419817 1 5.45
CHEMBL2419813 1 4.64
CHEMBL2419816 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2419814 IC50 = 7.0 nM 8.15
CHEMBL2419830 IC50 = 13.0 nM 7.89
CHEMBL2419811 IC50 = 16.0 nM 7.80
CHEMBL2419808 IC50 = 18.0 nM 7.75
CHEMBL2419819 IC50 = 20.0 nM 7.70
CHEMBL2419808 IC50 = 29.0 nM 7.54
CHEMBL2419827 IC50 = 30.0 nM 7.52
CHEMBL2419826 IC50 = 40.0 nM 7.40
CHEMBL2064166 IC50 = 50.0 nM 7.30
CHEMBL2419828 IC50 = 50.0 nM 7.30
CHEMBL2419810 IC50 = 60.0 nM 7.22
CHEMBL2419807 IC50 = 80.0 nM 7.10
CHEMBL2419821 IC50 = 90.0 nM 7.05
CHEMBL2419807 IC50 = 100.0 nM 7.00
CHEMBL2419829 IC50 = 140.0 nM 6.85
CHEMBL2419812 IC50 = 270.0 nM 6.57
CHEMBL2419825 IC50 = 270.0 nM 6.57
CHEMBL2417575 IC50 = 290.0 nM 6.54
CHEMBL2419815 IC50 = 310.0 nM 6.51
CHEMBL2419809 IC50 = 310.0 nM 6.51
CHEMBL2419822 IC50 = 390.0 nM 6.41
CHEMBL2419818 IC50 = 480.0 nM 6.32
CHEMBL2419824 IC50 = 760.0 nM 6.12
CHEMBL2419823 IC50 = 1170.0 nM 5.93
CHEMBL2419820 IC50 = 2500.0 nM 5.60
CHEMBL2419817 IC50 = 3530.0 nM 5.45
CHEMBL2419808 IC50 = 5100.0 nM 5.29
CHEMBL2419807 IC50 = 12200.0 nM 4.91
CHEMBL2419813 IC50 = 23000.0 nM 4.64
CHEMBL2419816 IC50 - -