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Assay Detail

CHEMBL2422696

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse mu opioid receptor expressed in CHO cells assessed as stimulation of [35S]-GTPgammaS binding after 1.5 hrs
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL2858)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Structure activity relationship studies of 17-cyclopropylmethyl-3,14β-dihydroxy-4,5α-epoxy-6α-(isoquinoline-3'-carboxamido)morphinan (NAQ) analogues as potent opioid receptor ligands: preliminary results on the role of electronic characteristics for affinity and function.
Yuan Y, Elbegdorj O, Beletskaya IO, Selley DE, Zhang Y.
Bioorg Med Chem Lett (2013) 23 : 5045 -5048
PubMed 23948248 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 8.55 9.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL19019 NALTREXONE 4.0 1 9.80
CHEMBL2417568 1 8.97
CHEMBL2419119 1 8.94
CHEMBL2419122 1 8.91
CHEMBL2419121 1 8.76
CHEMBL2419124 1 8.58
CHEMBL2419116 1 8.48
CHEMBL522201 1 8.48
CHEMBL2419118 1 8.34
CHEMBL2419123 1 8.22
CHEMBL2419125 1 8.14
CHEMBL2419120 1 7.85
CHEMBL2419117 1 7.71

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL19019 NALTREXONE EC50 = 0.16 nM 9.80
CHEMBL2417568 EC50 = 1.06 nM 8.97
CHEMBL2419119 EC50 = 1.14 nM 8.94
CHEMBL2419122 EC50 = 1.23 nM 8.91
CHEMBL2419121 EC50 = 1.74 nM 8.76
CHEMBL2419124 EC50 = 2.62 nM 8.58
CHEMBL2419116 EC50 = 3.32 nM 8.48
CHEMBL522201 EC50 = 3.3 nM 8.48
CHEMBL2419118 EC50 = 4.6 nM 8.34
CHEMBL2419123 EC50 = 6.0 nM 8.22
CHEMBL2419125 EC50 = 7.2 nM 8.14
CHEMBL2419120 EC50 = 14.1 nM 7.85
CHEMBL2419117 EC50 = 19.4 nM 7.71