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Assay Detail

CHEMBL2422901

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]ZM241385 from human A2A receptor expressed in HEK293 cell membranes after 60 mins by scintillation counting
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of (4E)-4-(4-substitutedbenzylideneamino)-3-substituted-2,3-dihydro-2-thioxothiazole-5-carbonitrile as novel A2A receptor antagonists.
Mishra CB, Sharma D, Prakash A, Kumari N, Kumar N, Luthra PM.
Bioorg Med Chem (2013) 21 : 6077 -6083
PubMed 23953686 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 9.14 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2419139 1 11.00
CHEMBL2419146 1 10.57
CHEMBL2419147 1 10.52
CHEMBL2419150 1 10.17
CHEMBL2419140 1 9.85
CHEMBL2419143 1 8.84
CHEMBL2419138 1 8.74
CHEMBL2419145 1 8.58
CHEMBL2419137 1 8.12
CHEMBL2419141 1 8.02
CHEMBL2419148 1 7.96
CHEMBL2419142 1 7.26
CHEMBL2419149 1 -
CHEMBL2419144 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2419139 Ki = 0.01 nM 11.00
CHEMBL2419146 Ki = 0.027 nM 10.57
CHEMBL2419147 Ki = 0.03 nM 10.52
CHEMBL2419150 Ki = 0.067 nM 10.17
CHEMBL2419140 Ki = 0.14 nM 9.85
CHEMBL2419143 Ki = 1.44 nM 8.84
CHEMBL2419138 Ki = 1.8 nM 8.74
CHEMBL2419145 Ki = 2.64 nM 8.58
CHEMBL2419137 Ki = 7.61 nM 8.12
CHEMBL2419141 Ki = 9.6 nM 8.02
CHEMBL2419148 Ki = 10.98 nM 7.96
CHEMBL2419142 Ki = 54.8 nM 7.26
CHEMBL2419149 Ki = 0.0042 nM -
CHEMBL2419144 Ki = 0.0047 nM -