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Assay Detail

CHEMBL2428207

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of glucocorticoid receptor ligand binding domain (unknown origin) transfected in human HeLa cells assessed as activation of NP-1 by GAL4 luciferase reporter gene assay
32
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL2034)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent and selective nonsteroidal indazolyl amide glucocorticoid receptor agonists.
Sheppeck JE, Gilmore JL, Xiao HY, Dhar TG, Nirschl D, Doweyko AM, Sack JS, Corbett MJ, Malley MF, Gougoutas JZ, Mckay L, Cunningham MD, Habte SF, Dodd JH, Nadler SG, Somerville JE, Barrish JC.
Bioorg Med Chem Lett (2013) 23 : 5442 -5447
PubMed 23953070 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 32 7.41 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2426624 2 10.00
CHEMBL384467 DEXAMETHASONE 4.0 1 8.35
CHEMBL2426662 1 8.24
CHEMBL2426665 1 8.12
CHEMBL2426623 2 7.70
CHEMBL2426656 1 6.88
CHEMBL2426648 1 6.88
CHEMBL2426633 1 6.73
CHEMBL2426658 1 6.68
CHEMBL2426645 1 6.59
CHEMBL2426644 1 6.36
CHEMBL1801004 1 6.28
CHEMBL2426632 1 -
CHEMBL2426631 1 -
CHEMBL2426630 1 -
CHEMBL2426657 1 -
CHEMBL2426655 1 -
CHEMBL2426654 1 -
CHEMBL2426653 1 -
CHEMBL2426652 1 -
CHEMBL2426651 1 -
CHEMBL2426650 1 -
CHEMBL2426649 1 -
CHEMBL2426646 1 -
CHEMBL2426664 1 -
CHEMBL2426663 1 -
CHEMBL2426661 1 -
CHEMBL2426660 1 -
CHEMBL2426659 1 -
CHEMBL2426647 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2426624 EC50 = 0.1 nM 10.00
CHEMBL384467 DEXAMETHASONE EC50 = 4.5 nM 8.35
CHEMBL2426662 EC50 = 5.8 nM 8.24
CHEMBL2426665 EC50 = 7.6 nM 8.12
CHEMBL2426623 EC50 = 20.0 nM 7.70
CHEMBL2426624 EC50 = 27.0 nM 7.57
CHEMBL2426648 EC50 = 132.0 nM 6.88
CHEMBL2426656 EC50 = 132.0 nM 6.88
CHEMBL2426633 EC50 = 188.0 nM 6.73
CHEMBL2426658 EC50 = 208.0 nM 6.68
CHEMBL2426645 EC50 = 256.0 nM 6.59
CHEMBL2426644 EC50 = 435.0 nM 6.36
CHEMBL1801004 EC50 = 526.0 nM 6.28
CHEMBL2426630 EC50 - -
CHEMBL2426655 EC50 > 10000.0 nM -
CHEMBL2426654 EC50 > 10000.0 nM -
CHEMBL2426653 EC50 > 10000.0 nM -
CHEMBL2426652 EC50 > 10000.0 nM -
CHEMBL2426651 EC50 > 10000.0 nM -
CHEMBL2426650 EC50 > 10000.0 nM -
CHEMBL2426649 EC50 > 10000.0 nM -
CHEMBL2426631 EC50 - -
CHEMBL2426647 EC50 - -
CHEMBL2426646 EC50 - -
CHEMBL2426632 EC50 - -
CHEMBL2426664 EC50 - -
CHEMBL2426663 EC50 - -
CHEMBL2426661 EC50 - -
CHEMBL2426660 EC50 - -
CHEMBL2426659 EC50 > 10000.0 nM -
CHEMBL2426657 EC50 - -
CHEMBL2426623 EC50 > 100.0 nM -