Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL2428849

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human thrombin amidolytic activity using D-Phe-Pip-Arg-pNA as substrate preincubated for 10 mins followed by substrate addition measured every 12 secs for 10 mins by spectrophotometric analysis
12
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prothrombin (CHEMBL204)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Thrombin inhibitory activity of some polyphenolic compounds.
Bijak M, Ziewiecki R, Saluk J, Ponczek M, Pawlaczyk I, Krotkiewski H, Wachowicz B, Nowak P.
Med Chem Res (2013) : 1 -14
PubMed 24610996 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Inhibition 6 - -
IC50 6 5.29 6.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL404515 CYANIDIN 2 6.60
CHEMBL50 QUERCETIN 3.0 2 5.82
CHEMBL9509 SILIBININ 3.0 2 4.60
CHEMBL2425137 CYANIN 2 4.12
CHEMBL583912 (-)-EPICATECHIN 2.0 2 -
CHEMBL311498 CIANIDANOL 4.0 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL404515 CYANIDIN IC50 = 250.0 nM 6.60
CHEMBL50 QUERCETIN IC50 = 1500.0 nM 5.82
CHEMBL9509 SILIBININ IC50 = 25000.0 nM 4.60
CHEMBL2425137 CYANIN IC50 = 75000.0 nM 4.12
CHEMBL583912 (-)-EPICATECHIN Inhibition - % -
CHEMBL311498 CIANIDANOL Inhibition - % -
CHEMBL2425137 CYANIN Inhibition - % -
CHEMBL9509 SILIBININ Inhibition - % -
CHEMBL50 QUERCETIN Inhibition - % -
CHEMBL404515 CYANIDIN Inhibition - % -
CHEMBL583912 (-)-EPICATECHIN IC50 = 150000.0 nM -
CHEMBL311498 CIANIDANOL IC50 = 125000.0 nM -