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Assay Detail

CHEMBL2433086

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His-FLAG-tagged BRD4 binding domain1 (unknown origin) binding to H4-TetraAc-biotin peptide after 20 mins by AlphaLISA
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bromodomain-containing protein 4 (CHEMBL1163125)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery, Design, and Optimization of Isoxazole Azepine BET Inhibitors.
Gehling VS, Hewitt MC, Vaswani RG, Leblanc Y, Côté A, Nasveschuk CG, Taylor AM, Harmange JC, Audia JE, Pardo E, Joshi S, Sandy P, Mertz JA, Sims RJ, Bergeron L, Bryant BM, Bellon S, Poy F, Jayaram H, Sankaranarayanan R, Yellapantula S, Bangalore Srinivasamurthy N, Birudukota S, Albrecht BK.
ACS Med Chem Lett (2013) 4 : 835 -840
PubMed 24900758 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.67 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2017291 1 7.75
CHEMBL1957266 1 7.62
CHEMBL2431090 1 7.58
CHEMBL2431078 1 7.54
CHEMBL2431079 1 7.48
CHEMBL2431091 1 7.44
CHEMBL2431080 1 7.38
CHEMBL2431082 1 7.14
CHEMBL2431081 1 7.14
CHEMBL2431076 1 7.13
CHEMBL2431093 1 7.07
CHEMBL1232461 MOLIBRESIB 2.0 1 6.92
CHEMBL2431094 1 6.72
CHEMBL2431074 1 6.72
CHEMBL2431073 1 6.70
CHEMBL2431077 1 6.60
CHEMBL2431075 1 6.60
CHEMBL2431089 1 6.54
CHEMBL2431085 1 6.52
CHEMBL2431086 1 5.96
CHEMBL2431092 1 5.68
CHEMBL2431083 1 5.60
CHEMBL2431084 1 5.24
CHEMBL2431087 1 5.21
CHEMBL2431088 1 4.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2017291 IC50 = 18.0 nM 7.75
CHEMBL1957266 IC50 = 24.0 nM 7.62
CHEMBL2431090 IC50 = 26.0 nM 7.58
CHEMBL2431078 IC50 = 29.0 nM 7.54
CHEMBL2431079 IC50 = 33.0 nM 7.48
CHEMBL2431091 IC50 = 36.0 nM 7.44
CHEMBL2431080 IC50 = 42.0 nM 7.38
CHEMBL2431082 IC50 = 73.0 nM 7.14
CHEMBL2431081 IC50 = 72.0 nM 7.14
CHEMBL2431076 IC50 = 74.0 nM 7.13
CHEMBL2431093 IC50 = 86.0 nM 7.07
CHEMBL1232461 MOLIBRESIB IC50 = 120.0 nM 6.92
CHEMBL2431094 IC50 = 190.0 nM 6.72
CHEMBL2431074 IC50 = 190.0 nM 6.72
CHEMBL2431073 IC50 = 200.0 nM 6.70
CHEMBL2431077 IC50 = 250.0 nM 6.60
CHEMBL2431075 IC50 = 250.0 nM 6.60
CHEMBL2431089 IC50 = 290.0 nM 6.54
CHEMBL2431085 IC50 = 300.0 nM 6.52
CHEMBL2431086 IC50 = 1100.0 nM 5.96
CHEMBL2431092 IC50 = 2100.0 nM 5.68
CHEMBL2431083 IC50 = 2500.0 nM 5.60
CHEMBL2431084 IC50 = 5700.0 nM 5.24
CHEMBL2431087 IC50 = 6200.0 nM 5.21
CHEMBL2431088 IC50 = 33000.0 nM 4.48