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Assay Detail

CHEMBL2438192

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at mouse OX1 receptor expressed in CHO cells assessed as inhibition of orexin A-induced Ca2+ accumulation after 1 hr by Fluo-4-AM staining-based FLIPR assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Orexin/Hypocretin receptor type 1 (CHEMBL2434819)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Identification of a novel series of orexin receptor antagonists with a distinct effect on sleep architecture for the treatment of insomnia.
Betschart C, Hintermann S, Behnke D, Cotesta S, Fendt M, Gee CE, Jacobson LH, Laue G, Ofner S, Chaudhari V, Badiger S, Pandit C, Wagner J, Hoyer D.
J Med Chem (2013) 56 : 7590 -7607
PubMed 23964859 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 7.56 8.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1083659 SUVOREXANT 4.0 1 8.77
CHEMBL2435402 1 6.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1083659 SUVOREXANT Ki = 1.698 nM 8.77
CHEMBL2435402 Ki = 457.09 nM 6.34