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Assay Detail

CHEMBL2438465

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of purified mouse COX-2 assessed as inhibition of PGE2/PGD2 formation preincubated for 3 mins before arachidonic acid substrate addition measured after 30 seconds by LC-MS-MS method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin G/H synthase 2 (CHEMBL4321)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Exploring the molecular determinants of substrate-selective inhibition of cyclooxygenase-2 by lumiracoxib.
Windsor MA, Valk PL, Xu S, Banerjee S, Marnett LJ.
Bioorg Med Chem Lett (2013) 23 : 5860 -5864
PubMed 24060487 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.78 6.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2435178 1 6.85
CHEMBL259218 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2435178 IC50 = 140.0 nM 6.85
CHEMBL259218 IC50 = 200.0 nM 6.70