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Assay Detail

CHEMBL3089200

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]epibatidine from rat alpha2beta2 nAChR expressed in HEK293 cells after 4 hrs
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha2/beta2 (CHEMBL1907592)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Design, synthesis and discovery of picomolar selective α4β2 nicotinic acetylcholine receptor ligands.
Yenugonda VM, Xiao Y, Levin ED, Rezvani AH, Tran T, Al-Muhtasib N, Sahibzada N, Xie T, Wells C, Slade S, Johnson JE, Dakshanamurthy S, Kong HS, Tomita Y, Liu Y, Paige M, Kellar KJ, Brown ML.
J Med Chem (2013) 56 : 8404 -8421
PubMed 24047231 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 9.81 10.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3086994 1 10.54
CHEMBL3086992 1 10.35
CHEMBL3086982 1 10.28
CHEMBL3086984 1 10.28
CHEMBL3086995 1 10.20
CHEMBL3086985 1 10.19
CHEMBL3086993 1 10.09
CHEMBL2024096 1 10.06
CHEMBL3086986 1 10.04
CHEMBL3086991 1 9.96
CHEMBL3086983 1 9.72
CHEMBL3086989 1 9.72
CHEMBL1076903 VARENICLINE 4.0 1 9.66
CHEMBL96201 1 9.66
CHEMBL3086996 1 9.55
CHEMBL3086990 1 8.54
CHEMBL3 NICOTINE 4.0 1 7.92
CHEMBL3086987 1 -
CHEMBL3086988 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3086994 Ki = 0.029 nM 10.54
CHEMBL3086992 Ki = 0.045 nM 10.35
CHEMBL3086982 Ki = 0.053 nM 10.28
CHEMBL3086984 Ki = 0.053 nM 10.28
CHEMBL3086995 Ki = 0.063 nM 10.20
CHEMBL3086985 Ki = 0.064 nM 10.19
CHEMBL3086993 Ki = 0.081 nM 10.09
CHEMBL2024096 Ki = 0.087 nM 10.06
CHEMBL3086986 Ki = 0.091 nM 10.04
CHEMBL3086991 Ki = 0.11 nM 9.96
CHEMBL3086983 Ki = 0.19 nM 9.72
CHEMBL3086989 Ki = 0.19 nM 9.72
CHEMBL1076903 VARENICLINE Ki = 0.22 nM 9.66
CHEMBL96201 Ki = 0.22 nM 9.66
CHEMBL3086996 Ki = 0.28 nM 9.55
CHEMBL3086990 Ki = 2.9 nM 8.54
CHEMBL3 NICOTINE Ki = 12.0 nM 7.92
CHEMBL3086987 Ki > 100000.0 nM -
CHEMBL3086988 Ki > 5000.0 nM -