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Assay Detail

CHEMBL3091212

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Wistar rat testicular C17,20-lyase using [3H]17-hydroxyprogesterone as substrate preincubated for 20 mins
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Steroid 17-alpha-hydroxylase/17,20 lyase (CHEMBL4430)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

An efficient approach to novel 17-5'-(1',2',4')-oxadiazolyl androstenes via the cyclodehydration of cytotoxic O-steroidacylamidoximes, and an evaluation of their inhibitory action on 17α-hydroxylase/C₁₇,₂₀-lyase.
Kovács D, Wölfling J, Szabó N, Szécsi M, Kovács I, Zupkó I, Frank E.
Eur J Med Chem (2013) 70 : 649 -660
PubMed 24211641 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.87 7.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL254328 ABIRATERONE 3.0 1 7.90
CHEMBL157101 KETOCONAZOLE 4.0 1 6.50
CHEMBL3087962 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL254328 ABIRATERONE IC50 = 12.5 nM 7.90
CHEMBL157101 KETOCONAZOLE IC50 = 320.0 nM 6.50
CHEMBL3087962 IC50 = 600.0 nM 6.22