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Assay Detail

CHEMBL3094808

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human erythrocytic AChE using acetylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition measured every 1 min by Ellman method
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibition of cholinesterase and monoamine oxidase-B activity by Tacrine-Homoisoflavonoid hybrids.
Sun Y, Chen J, Chen X, Huang L, Li X.
Bioorg Med Chem (2013) 21 : 7406 -7417
PubMed 24128814 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.85 7.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3093173 1 7.01
CHEMBL3093180 1 6.98
CHEMBL3093175 1 6.92
CHEMBL3093174 1 6.80
CHEMBL95 TACRINE 4.0 1 6.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3093173 IC50 = 98.0 nM 7.01
CHEMBL3093180 IC50 = 105.3 nM 6.98
CHEMBL3093175 IC50 = 121.6 nM 6.92
CHEMBL3093174 IC50 = 156.8 nM 6.80
CHEMBL95 TACRINE IC50 = 285.0 nM 6.54