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Assay Detail

CHEMBL3095035

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Uncompetitive inhibition of porcine kidney TNAP assessed as para nitrophenylphosphate conversion to p-nitrophenolate at pH 7.8 by Michaelis-Menten plot analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Tissue Kidney
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Inhibitors of tissue-nonspecific alkaline phosphatase: design, synthesis, kinetics, biomineralization and cellular tests.
Debray J, Chang L, Marquès S, Pellet-Rostaing S, Le Duy D, Mebarek S, Buchet R, Magne D, Popowycz F, Lemaire M.
Bioorg Med Chem (2013) 21 : 7981 -7987
PubMed 24183741 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 5.22 5.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3092293 1 5.89
CHEMBL3092295 1 5.41
CHEMBL3092296 1 5.30
CHEMBL3092294 1 4.99
CHEMBL1454 LEVAMISOLE 4.0 1 4.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3092293 Ki = 1300.0 nM 5.89
CHEMBL3092295 Ki = 3900.0 nM 5.41
CHEMBL3092296 Ki = 5000.0 nM 5.30
CHEMBL3092294 Ki = 10300.0 nM 4.99
CHEMBL1454 LEVAMISOLE Ki = 30500.0 nM 4.52