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Assay Detail

CHEMBL3096293

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human BCRP expressed in MDCK2 cells assessed as Hoechst 33342 accumulation treated 30 mins before Hoechst 33342 addition measured up to 120 mins by fluorescence assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MDCK-II
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Investigation of quinazolines as inhibitors of breast cancer resistance protein (ABCG2).
Juvale K, Gallus J, Wiese M.
Bioorg Med Chem (2013) 21 : 7858 -7873
PubMed 24184213 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 6.13 7.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3092485 1 7.12
CHEMBL3092490 1 6.85
CHEMBL1360562 1 6.82
CHEMBL3092488 1 6.72
CHEMBL488910 1 6.60
CHEMBL3092489 1 6.58
CHEMBL3092482 1 6.54
CHEMBL3092486 1 6.52
CHEMBL3092487 1 6.52
CHEMBL3092500 1 6.48
CHEMBL3092483 1 6.38
CHEMBL3092491 1 6.33
CHEMBL3092499 1 6.32
CHEMBL3092484 1 6.19
CHEMBL1999832 1 6.17
CHEMBL3092498 1 6.10
CHEMBL3092496 1 6.09
CHEMBL92824 1 6.03
CHEMBL2333998 1 5.97
CHEMBL290096 1 5.97
CHEMBL3092493 1 5.95
CHEMBL3092492 1 5.93
CHEMBL29197 1 5.76
CHEMBL3092501 1 5.76
CHEMBL3092481 1 5.70
CHEMBL316064 1 5.62
CHEMBL3092497 1 5.54
CHEMBL939 GEFITINIB 4.0 1 5.50
CHEMBL3092495 1 5.42
CHEMBL3092494 1 5.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3092485 IC50 = 76.0 nM 7.12
CHEMBL3092490 IC50 = 140.0 nM 6.85
CHEMBL1360562 IC50 = 150.0 nM 6.82
CHEMBL3092488 IC50 = 190.0 nM 6.72
CHEMBL488910 IC50 = 250.0 nM 6.60
CHEMBL3092489 IC50 = 260.0 nM 6.58
CHEMBL3092482 IC50 = 290.0 nM 6.54
CHEMBL3092486 IC50 = 300.0 nM 6.52
CHEMBL3092487 IC50 = 300.0 nM 6.52
CHEMBL3092500 IC50 = 330.0 nM 6.48
CHEMBL3092483 IC50 = 420.0 nM 6.38
CHEMBL3092491 IC50 = 470.0 nM 6.33
CHEMBL3092499 IC50 = 480.0 nM 6.32
CHEMBL3092484 IC50 = 640.0 nM 6.19
CHEMBL1999832 IC50 = 670.0 nM 6.17
CHEMBL3092498 IC50 = 800.0 nM 6.10
CHEMBL3092496 IC50 = 810.0 nM 6.09
CHEMBL92824 IC50 = 940.0 nM 6.03
CHEMBL2333998 IC50 = 1060.0 nM 5.97
CHEMBL290096 IC50 = 1080.0 nM 5.97
CHEMBL3092493 IC50 = 1130.0 nM 5.95
CHEMBL3092492 IC50 = 1180.0 nM 5.93
CHEMBL29197 IC50 = 1730.0 nM 5.76
CHEMBL3092501 IC50 = 1740.0 nM 5.76
CHEMBL3092481 IC50 = 1990.0 nM 5.70
CHEMBL316064 IC50 = 2400.0 nM 5.62
CHEMBL3092497 IC50 = 2920.0 nM 5.54
CHEMBL939 GEFITINIB IC50 = 3190.0 nM 5.50
CHEMBL3092495 IC50 = 3800.0 nM 5.42
CHEMBL3092494 IC50 = 4790.0 nM 5.32
CHEMBL100714 IC50 = 7380.0 nM 5.13