Assay Detail
Binding
CHEMBL3100280
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant AKT1 (unknown origin) using serine/threonine 06 peptide as substrate after 1 hr by FRET based Z'-LYTE assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
RAC-alpha serine/threonine-protein kinase (CHEMBL4282) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Preparation and evaluation of deconstruction analogues of 7-deoxykalafungin as AKT kinase inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 5.80 | 7.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL388978 | STAUROSPORINE | — | 1 | 7.96 |
| CHEMBL475400 | — | — | 1 | 7.36 |
| CHEMBL522996 | — | — | 1 | 6.55 |
| CHEMBL3099612 | — | — | 1 | 5.53 |
| CHEMBL55934 | NAPHTHOQUINONE | — | 1 | 4.96 |
| CHEMBL3099615 | — | — | 1 | 4.91 |
| CHEMBL3099614 | — | — | 1 | 4.86 |
| CHEMBL3099613 | — | — | 1 | 4.30 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL388978 | STAUROSPORINE | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL475400 | — | IC50 | = | 44.0 | nM | 7.36 |
| CHEMBL522996 | — | IC50 | = | 280.0 | nM | 6.55 |
| CHEMBL3099612 | — | IC50 | = | 2940.0 | nM | 5.53 |
| CHEMBL55934 | NAPHTHOQUINONE | IC50 | = | 10980.0 | nM | 4.96 |
| CHEMBL3099615 | — | IC50 | = | 12270.0 | nM | 4.91 |
| CHEMBL3099614 | — | IC50 | = | 13670.0 | nM | 4.86 |
| CHEMBL3099613 | — | IC50 | = | 50000.0 | nM | 4.30 |