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Assay Detail

CHEMBL3105977

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat recombinant 6xHis-tagged DYRK1A catalytic domain (1 to 502) expressed in Escherichia coli BL21 (DE3) using N-terminal fluorescein coupled KISGRLSPIMTEQ as substrate after 30 mins by HPLC analysis in presence of 50 uM ATP
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity tyrosine-phosphorylation-regulated kinase 1A (CHEMBL5508)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Development of DANDYs, new 3,5-diaryl-7-azaindoles demonstrating potent DYRK1A kinase inhibitory activity.
Gourdain S, Dairou J, Denhez C, Bui LC, Rodrigues-Lima F, Janel N, Delabar JM, Cariou K, Dodd RH.
J Med Chem (2013) 56 : 9569 -9585
PubMed 24188002 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.38 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3102952 1 8.52
CHEMBL3102951 1 7.93
CHEMBL3102955 1 7.91
CHEMBL3102953 1 7.84
CHEMBL3102950 1 7.64
CHEMBL3102954 1 7.41
CHEMBL3102956 1 6.98
CHEMBL3102957 1 6.82
CHEMBL3102942 1 6.80
CHEMBL3102944 1 6.55
CHEMBL3102935 1 6.48
CHEMBL3102859 1 6.39
CHEMBL3102945 1 6.34
CHEMBL3102938 1 6.02
CHEMBL3102934 1 6.02
CHEMBL3102940 1 5.93
CHEMBL3102947 1 5.78
CHEMBL3102943 1 5.76
CHEMBL3102937 1 5.57
CHEMBL3102946 1 5.54
CHEMBL3102941 1 5.51
CHEMBL3102939 1 5.45
CHEMBL3102936 1 5.13
CHEMBL3102949 1 4.83
CHEMBL3102948 1 4.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3102952 IC50 = 3.0 nM 8.52
CHEMBL3102951 IC50 = 11.7 nM 7.93
CHEMBL3102955 IC50 = 12.4 nM 7.91
CHEMBL3102953 IC50 = 14.3 nM 7.84
CHEMBL3102950 IC50 = 23.1 nM 7.64
CHEMBL3102954 IC50 = 39.1 nM 7.41
CHEMBL3102956 IC50 = 105.2 nM 6.98
CHEMBL3102957 IC50 = 150.0 nM 6.82
CHEMBL3102942 IC50 = 160.0 nM 6.80
CHEMBL3102944 IC50 = 280.0 nM 6.55
CHEMBL3102935 IC50 = 330.0 nM 6.48
CHEMBL3102859 IC50 = 410.0 nM 6.39
CHEMBL3102945 IC50 = 460.0 nM 6.34
CHEMBL3102938 IC50 = 960.0 nM 6.02
CHEMBL3102934 IC50 = 960.0 nM 6.02
CHEMBL3102940 IC50 = 1170.0 nM 5.93
CHEMBL3102947 IC50 = 1670.0 nM 5.78
CHEMBL3102943 IC50 = 1740.0 nM 5.76
CHEMBL3102937 IC50 = 2700.0 nM 5.57
CHEMBL3102946 IC50 = 2880.0 nM 5.54
CHEMBL3102941 IC50 = 3080.0 nM 5.51
CHEMBL3102939 IC50 = 3590.0 nM 5.45
CHEMBL3102936 IC50 = 7490.0 nM 5.13
CHEMBL3102949 IC50 = 14630.0 nM 4.83
CHEMBL3102948 IC50 = 57780.0 nM 4.24