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Assay Detail

CHEMBL3106641

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production administered 1 hr prior to LPS-challenge measured after 24 hrs by EIA
7
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis of tricyclic fused coumarin sulfonates and their inhibitory effects on LPS-induced nitric oxide and PGE2 productions in RAW 264.7 macrophages.
Jang HL, El-Gamal MI, Choi HE, Choi HY, Lee KT, Oh CH.
Bioorg Med Chem Lett (2014) 24 : 571 -575
PubMed 24360561 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 8.04 8.40
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3103243 1 8.40
CHEMBL3103247 1 8.22
CHEMBL3103240 1 8.19
CHEMBL3103246 1 8.17
CHEMBL7162 NS-398 1 8.15
CHEMBL286738 IROSUSTAT 2.0 2 7.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3103243 IC50 = 4.02 nM 8.40
CHEMBL3103247 IC50 = 6.04 nM 8.22
CHEMBL3103240 IC50 = 6.45 nM 8.19
CHEMBL3103246 IC50 = 6.75 nM 8.17
CHEMBL7162 NS-398 IC50 = 7.01 nM 8.15
CHEMBL286738 IROSUSTAT IC50 = 81.04 nM 7.09
CHEMBL286738 IROSUSTAT Inhibition - % -