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Assay Detail

CHEMBL3107209

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Uncompetitive inhibition of soybean LOX-1 using linoleic acid as substrate preincubated for 5 mins followed by substrate addition by Lineweaver-Burk plot analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Glycine max
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Seed linoleate 13S-lipoxygenase-1 (CHEMBL4586)
Type SINGLE PROTEIN
Organism Glycine max

Publication

Inhibition of LOX by flavonoids: a structure-activity relationship study.
Ribeiro D, Freitas M, Tomé SM, Silva AM, Porto G, Cabrita EJ, Marques MM, Fernandes E.
Eur J Med Chem (2014) 72 : 137 -145
PubMed 24368208 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 4.39 4.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL243677 1 4.75
CHEMBL457821 1 4.02
CHEMBL50 QUERCETIN 3.0 1 -
CHEMBL151 LUTEOLIN 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL243677 Ki = 18000.0 nM 4.75
CHEMBL457821 Ki = 95400.0 nM 4.02
CHEMBL50 QUERCETIN Ki = 106700.0 nM -
CHEMBL151 LUTEOLIN Ki = 121700.0 nM -