Assay Detail
Functional
CHEMBL3110490
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of rat TAAR1 transmembrane domain 7 Asn287(7.39)Tyr mutant expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Trace amine-associated receptor 1 (CHEMBL3833) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Exploring the determinants of trace amine-associated receptor 1's functional selectivity for the stereoisomers of amphetamine and methamphetamine.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 6.92 | 7.86 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL612 | DEXTROAMPHETAMINE | 4.0 | 1 | 7.86 |
| CHEMBL19393 | LEVAMFETAMINE | 4.0 | 1 | 6.77 |
| CHEMBL610 | PHENETHYLAMINE | — | 1 | 6.77 |
| CHEMBL1201201 | METHAMPHETAMINE | 4.0 | 1 | 6.70 |
| CHEMBL1927030 | LEVMETAMFETAMINE | 2.0 | 1 | 6.48 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL612 | DEXTROAMPHETAMINE | EC50 | = | 13.9 | nM | 7.86 |
| CHEMBL19393 | LEVAMFETAMINE | EC50 | = | 169.9 | nM | 6.77 |
| CHEMBL610 | PHENETHYLAMINE | EC50 | = | 170.6 | nM | 6.77 |
| CHEMBL1201201 | METHAMPHETAMINE | EC50 | = | 201.8 | nM | 6.70 |
| CHEMBL1927030 | LEVMETAMFETAMINE | EC50 | = | 330.4 | nM | 6.48 |