Assay Detail
Functional
CHEMBL3110494
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of rat wild type TAAR1 expressed in HEK293 cells assessed as stimulation of cAMP production after 1 hr by chemiluminescent assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Trace amine-associated receptor 1 (CHEMBL3833) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Exploring the determinants of trace amine-associated receptor 1's functional selectivity for the stereoisomers of amphetamine and methamphetamine.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 7.12 | 7.47 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL19393 | LEVAMFETAMINE | 4.0 | 1 | 7.47 |
| CHEMBL610 | PHENETHYLAMINE | — | 1 | 7.39 |
| CHEMBL612 | DEXTROAMPHETAMINE | 4.0 | 1 | 7.25 |
| CHEMBL1201201 | METHAMPHETAMINE | 4.0 | 1 | 6.88 |
| CHEMBL1927030 | LEVMETAMFETAMINE | 2.0 | 1 | 6.63 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL19393 | LEVAMFETAMINE | EC50 | = | 33.9 | nM | 7.47 |
| CHEMBL610 | PHENETHYLAMINE | EC50 | = | 40.33 | nM | 7.39 |
| CHEMBL612 | DEXTROAMPHETAMINE | EC50 | = | 55.6 | nM | 7.25 |
| CHEMBL1201201 | METHAMPHETAMINE | EC50 | = | 133.3 | nM | 6.88 |
| CHEMBL1927030 | LEVMETAMFETAMINE | EC50 | = | 233.3 | nM | 6.63 |