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Assay Detail

CHEMBL3111918

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human factor D expressed in Escherichia coli using Z-Lys-thiobenzyl and 2,4-dinitrobenzenesulfonyl-fluoresceine as substrate preincubated for 1 hr followed by substrate addition by spectrofluorimetric analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Complement factor D (CHEMBL2176771)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors of factor d may provide a treatment for age-related macular degeneration.
Abdel-Magid AF.
ACS Med Chem Lett (2014) 5 : 106 -107
PubMed 24900799 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 8.48 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3109655 1 8.70
CHEMBL3109654 1 8.52
CHEMBL3109653 1 8.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3109655 IC50 = 2.0 nM 8.70
CHEMBL3109654 IC50 = 3.0 nM 8.52
CHEMBL3109653 IC50 = 6.0 nM 8.22