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Assay Detail

CHEMBL3119479

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human thrombin using S-2366 as substrate preincubated for 300 seconds followed by substrate addition measured after 40 mins by spectrophotometric analysis
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prothrombin (CHEMBL204)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and SAR of a series of activated protein C (APC) inhibitors with selectivity against thrombin for the treatment of haemophilia.
Bach P, Knerr L, Fjellström O, Hansson K, Mattsson C, Gustafsson D.
Bioorg Med Chem Lett (2014) 24 : 821 -827
PubMed 24418773 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.37 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL266349 MELAGATRAN 4.0 1 8.40
CHEMBL3115901 1 6.05
CHEMBL3115904 1 5.89
CHEMBL3115905 1 5.72
CHEMBL3115903 1 5.43
CHEMBL3115902 1 5.42
CHEMBL3115900 1 5.41
CHEMBL3115899 1 5.28
CHEMBL3115906 1 5.28
CHEMBL3115897 1 5.21
CHEMBL3115907 1 5.16
CHEMBL3115898 1 4.92
CHEMBL3115893 1 4.92
CHEMBL3115894 1 4.66
CHEMBL3115892 1 4.58
CHEMBL3115896 1 4.55
CHEMBL215295 1 4.44
CHEMBL3115891 1 -
CHEMBL3115895 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL266349 MELAGATRAN IC50 = 4.0 nM 8.40
CHEMBL3115901 IC50 = 900.0 nM 6.05
CHEMBL3115904 IC50 = 1300.0 nM 5.89
CHEMBL3115905 IC50 = 1900.0 nM 5.72
CHEMBL3115903 IC50 = 3700.0 nM 5.43
CHEMBL3115902 IC50 = 3800.0 nM 5.42
CHEMBL3115900 IC50 = 3900.0 nM 5.41
CHEMBL3115899 IC50 = 5200.0 nM 5.28
CHEMBL3115906 IC50 = 5200.0 nM 5.28
CHEMBL3115897 IC50 = 6100.0 nM 5.21
CHEMBL3115907 IC50 = 7000.0 nM 5.16
CHEMBL3115898 IC50 = 12000.0 nM 4.92
CHEMBL3115893 IC50 = 12000.0 nM 4.92
CHEMBL3115894 IC50 = 22000.0 nM 4.66
CHEMBL3115892 IC50 = 26000.0 nM 4.58
CHEMBL3115896 IC50 = 28000.0 nM 4.55
CHEMBL215295 IC50 = 36000.0 nM 4.44
CHEMBL3115891 IC50 > 44000.0 nM -
CHEMBL3115895 IC50 > 44000.0 nM -