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Assay Detail

CHEMBL3124385

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human serum BChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured after 5 mins by Ellman's method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL1914)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,2,3,4-Tetrahydrobenzo[h][1,6]naphthyridines as a new family of potent peripheral-to-midgorge-site inhibitors of acetylcholinesterase: synthesis, pharmacological evaluation and mechanistic studies.
Di Pietro O, Viayna E, Vicente-García E, Bartolini M, Ramón R, Juárez-Jiménez J, Clos MV, Pérez B, Andrisano V, Luque FJ, Lavilla R, Muñoz-Torrero D.
Eur J Med Chem (2014) 73 : 141 -152
PubMed 24389509 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.94 7.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL95 TACRINE 4.0 1 7.34
CHEMBL3122167 1 6.04
CHEMBL3122168 1 5.86
CHEMBL3122169 1 5.59
CHEMBL345124 PROPIDIUM 1 4.88
CHEMBL3122150 1 -
CHEMBL3122145 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL95 TACRINE IC50 = 46.0 nM 7.34
CHEMBL3122167 IC50 = 920.0 nM 6.04
CHEMBL3122168 IC50 = 1370.0 nM 5.86
CHEMBL3122169 IC50 = 2590.0 nM 5.59
CHEMBL345124 PROPIDIUM IC50 = 13200.0 nM 4.88
CHEMBL3122150 IC50 - -
CHEMBL3122145 IC50 - -