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Assay Detail

CHEMBL3132469

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human GST-thrombin-tagged MDM2 assessed as inhibition of interaction with human p53 after 1 hr by HTRF assay in presence of 15% human serum
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

E3 ubiquitin-protein ligase Mdm2 (CHEMBL5023)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of AMG 232, a potent, selective, and orally bioavailable MDM2-p53 inhibitor in clinical development.
Sun D, Li Z, Rew Y, Gribble M, Bartberger MD, Beck HP, Canon J, Chen A, Chen X, Chow D, Deignan J, Duquette J, Eksterowicz J, Fisher B, Fox BM, Fu J, Gonzalez AZ, Gonzalez-Lopez De Turiso F, Houze JB, Huang X, Jiang M, Jin L, Kayser F, Liu JJ, Lo MC, Long AM, Lucas B, McGee LR, McIntosh J, Mihalic J, Oliner JD, Osgood T, Peterson ML, Roveto P, Saiki AY, Shaffer P, Toteva M, Wang Y, Wang YC, Wortman S, Yakowec P, Yan X, Ye Q, Yu D, Yu M, Zhao X, Zhou J, Zhu J, Olson SH, Medina JC.
J Med Chem (2014) 57 : 1454 -1472
PubMed 24456472 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 8.40 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3125527 1 8.96
CHEMBL3125529 1 8.96
CHEMBL3125531 1 8.89
CHEMBL3125526 1 8.85
CHEMBL3125530 1 8.80
CHEMBL3125524 1 8.80
CHEMBL3125522 1 8.80
CHEMBL3125525 1 8.77
CHEMBL3125528 1 8.74
CHEMBL3125523 1 8.47
CHEMBL3125532 1 8.42
CHEMBL2059435 1 8.39
CHEMBL3125705 1 8.33
CHEMBL3125706 1 8.25
CHEMBL3125707 1 8.19
CHEMBL3125709 1 7.66
CHEMBL3125708 1 7.66
CHEMBL3125703 1 7.50
CHEMBL3125704 1 7.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3125527 IC50 = 1.1 nM 8.96
CHEMBL3125529 IC50 = 1.1 nM 8.96
CHEMBL3125531 IC50 = 1.3 nM 8.89
CHEMBL3125526 IC50 = 1.4 nM 8.85
CHEMBL3125530 IC50 = 1.6 nM 8.80
CHEMBL3125524 IC50 = 1.6 nM 8.80
CHEMBL3125522 IC50 = 1.6 nM 8.80
CHEMBL3125525 IC50 = 1.7 nM 8.77
CHEMBL3125528 IC50 = 1.8 nM 8.74
CHEMBL3125523 IC50 = 3.4 nM 8.47
CHEMBL3125532 IC50 = 3.8 nM 8.42
CHEMBL2059435 IC50 = 4.1 nM 8.39
CHEMBL3125705 IC50 = 4.7 nM 8.33
CHEMBL3125706 IC50 = 5.6 nM 8.25
CHEMBL3125707 IC50 = 6.5 nM 8.19
CHEMBL3125709 IC50 = 22.0 nM 7.66
CHEMBL3125708 IC50 = 22.0 nM 7.66
CHEMBL3125703 IC50 = 32.0 nM 7.50
CHEMBL3125704 IC50 = 62.4 nM 7.21