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Assay Detail

CHEMBL3134976

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Norwalk virus recombinant His6-tagged protease using EDANS-EPDFHLQGPEDLAK-Dabcyl as substrate preincubated for 45 mins followed by substrate addition measured every 1 min for 30 mins by FRET analysis
16
Total Activities
16
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Norwalk virus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis, Activity and Structure-Activity Relationship of Noroviral Protease Inhibitors.
Deng L, Muhaxhiri Z, Estes MK, Palzkill T, Prasad BV, Song Y.
Medchemcomm (2013) 4 : 1354 -1359
PubMed 24244836 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 5.76 7.12
Inhibition 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3134264 1 7.12
CHEMBL419332 1 6.92
CHEMBL3134256 1 6.51
CHEMBL3134254 1 6.23
CHEMBL3134263 1 5.85
CHEMBL95007 1 5.82
CHEMBL3134258 1 5.80
CHEMBL3134255 1 5.72
CHEMBL3134262 1 5.29
CHEMBL3134257 1 5.09
CHEMBL3134260 1 4.41
CHEMBL3134259 1 4.34
CHEMBL94557 1 -
CHEMBL19648 1 -
CHEMBL20668 1 -
CHEMBL3134261 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3134264 Ki = 75.0 nM 7.12
CHEMBL419332 Ki = 120.0 nM 6.92
CHEMBL3134256 Ki = 310.0 nM 6.51
CHEMBL3134254 Ki = 590.0 nM 6.23
CHEMBL3134263 Ki = 1400.0 nM 5.85
CHEMBL95007 Ki = 1500.0 nM 5.82
CHEMBL3134258 Ki = 1600.0 nM 5.80
CHEMBL3134255 Ki = 1900.0 nM 5.72
CHEMBL3134262 Ki = 5100.0 nM 5.29
CHEMBL3134257 Ki = 8200.0 nM 5.09
CHEMBL3134260 Ki = 39000.0 nM 4.41
CHEMBL3134259 Ki = 46000.0 nM 4.34
CHEMBL94557 Inhibition - % -
CHEMBL19648 Inhibition - % -
CHEMBL20668 Inhibition - % -
CHEMBL3134261 Ki = 150000.0 nM -