Assay Detail
Binding
CHEMBL3224412
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Competitive reversible inhibition of Strepotomyces coelicolor type 2 dehydroquinase expressed in Escherichia coli BL21 (DE3) cells assessed as enone-carboxylate chromophore of 3-dehydroshikimate formation using 3-dehydroquinate as substrate preincubated with enzyme for 3 mins prior to substrate addition by UV spectrophotometric assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Streptomyces coelicolor |
| Cell Type | BL21-DE3 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
3-dehydroquinate dehydratase (CHEMBL5276) ↗| Type | SINGLE PROTEIN |
| Organism | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
Publication
Rapid assembly of potent type II dehydroquinase inhibitors viaClick chemistry
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 8 | 7.25 | 8.24 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1823559 | — | — | 1 | 8.24 |
| CHEMBL3221803 | — | — | 1 | 8.24 |
| CHEMBL3221799 | — | — | 1 | 7.54 |
| CHEMBL3221802 | — | — | 1 | 7.32 |
| CHEMBL3221805 | — | — | 1 | 7.14 |
| CHEMBL3221800 | — | — | 1 | 7.11 |
| CHEMBL3221804 | — | — | 1 | 6.66 |
| CHEMBL3221801 | — | — | 1 | 5.76 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1823559 | — | Ki | = | 5.7 | nM | 8.24 |
| CHEMBL3221803 | — | Ki | = | 5.7 | nM | 8.24 |
| CHEMBL3221799 | — | Ki | = | 29.0 | nM | 7.54 |
| CHEMBL3221802 | — | Ki | = | 48.0 | nM | 7.32 |
| CHEMBL3221805 | — | Ki | = | 73.0 | nM | 7.14 |
| CHEMBL3221800 | — | Ki | = | 78.0 | nM | 7.11 |
| CHEMBL3221804 | — | Ki | = | 217.0 | nM | 6.66 |
| CHEMBL3221801 | — | Ki | = | 1720.0 | nM | 5.76 |