Assay Detail
Binding
CHEMBL3239456
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of GST-tagged Escherichia coli HPPK expressed in Escherichia coli using 6-hydroxymethyl-7,8-dihydropterin hydrochloride as substrate after 20 mins by luminescence assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (CHEMBL3217379) ↗| Type | SINGLE PROTEIN |
| Organism | Escherichia coli (strain K12) |
Publication
The identification, analysis and structure-based development of novel inhibitors of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 4.18 | 4.36 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3233207 | — | — | 1 | 4.36 |
| CHEMBL3233208 | — | — | 1 | 4.29 |
| CHEMBL3233203 | — | — | 1 | 4.19 |
| CHEMBL3233205 | — | — | 1 | 4.10 |
| CHEMBL3233204 | — | — | 1 | 4.09 |
| CHEMBL3233206 | — | — | 1 | 4.06 |
| CHEMBL3233201 | — | — | 1 | - |
| CHEMBL3233202 | — | — | 1 | - |
| CHEMBL3233209 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3233207 | — | IC50 | = | 44000.0 | nM | 4.36 |
| CHEMBL3233208 | — | IC50 | = | 51000.0 | nM | 4.29 |
| CHEMBL3233203 | — | IC50 | = | 64000.0 | nM | 4.19 |
| CHEMBL3233205 | — | IC50 | = | 79000.0 | nM | 4.10 |
| CHEMBL3233204 | — | IC50 | = | 82000.0 | nM | 4.09 |
| CHEMBL3233206 | — | IC50 | = | 88000.0 | nM | 4.06 |
| CHEMBL3233201 | — | IC50 | = | 139000.0 | nM | - |
| CHEMBL3233202 | — | IC50 | = | 254000.0 | nM | - |
| CHEMBL3233209 | — | IC50 | = | 243000.0 | nM | - |