Assay Detail
Binding
CHEMBL3239457
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Binding affinity to GST-tagged Escherichia coli HPPK expressed in Escherichia coli by SPR assay based alternative equilibrium affinity method in presence of AMP-CPP
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (CHEMBL3217379) ↗| Type | SINGLE PROTEIN |
| Organism | Escherichia coli (strain K12) |
Publication
The identification, analysis and structure-based development of novel inhibitors of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 14 | 4.86 | 5.24 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3233204 | — | — | 1 | 5.24 |
| CHEMBL3233208 | — | — | 1 | 5.23 |
| CHEMBL3233203 | — | — | 1 | 5.17 |
| CHEMBL506639 | ISOGUANINE | — | 1 | 5.12 |
| CHEMBL3233201 | — | — | 1 | 5.01 |
| CHEMBL3233205 | — | — | 1 | 4.95 |
| CHEMBL3233207 | — | — | 1 | 4.84 |
| CHEMBL727 | THIOGUANINE | 4.0 | 1 | 4.75 |
| CHEMBL3233216 | — | — | 1 | 4.75 |
| CHEMBL3233209 | — | — | 1 | 4.72 |
| CHEMBL3233202 | — | — | 1 | 4.54 |
| CHEMBL219568 | GUANINE | 3.0 | 1 | 4.42 |
| CHEMBL3233206 | — | — | 1 | 4.41 |
| CHEMBL178006 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3233204 | — | Kd | = | 5800.0 | nM | 5.24 |
| CHEMBL3233208 | — | Kd | = | 5900.0 | nM | 5.23 |
| CHEMBL3233203 | — | Kd | = | 6700.0 | nM | 5.17 |
| CHEMBL506639 | ISOGUANINE | Kd | = | 7500.0 | nM | 5.12 |
| CHEMBL3233201 | — | Kd | = | 9700.0 | nM | 5.01 |
| CHEMBL3233205 | — | Kd | = | 11200.0 | nM | 4.95 |
| CHEMBL3233207 | — | Kd | = | 14500.0 | nM | 4.84 |
| CHEMBL727 | THIOGUANINE | Kd | = | 18000.0 | nM | 4.75 |
| CHEMBL3233216 | — | Kd | = | 18000.0 | nM | 4.75 |
| CHEMBL3233209 | — | Kd | = | 18900.0 | nM | 4.72 |
| CHEMBL3233202 | — | Kd | = | 29000.0 | nM | 4.54 |
| CHEMBL219568 | GUANINE | Kd | = | 38000.0 | nM | 4.42 |
| CHEMBL3233206 | — | Kd | = | 39000.0 | nM | 4.41 |
| CHEMBL178006 | — | Kd | > | 100000.0 | nM | - |