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Assay Detail

CHEMBL3240701

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of biotin-tagged human GKRP/fluorescein-tagged GK interaction preincubated for 20 mins prior to GK addition measured after 2 to 4 hrs by AlphaScreen assay
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucokinase regulatory protein (CHEMBL1075152)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small molecule disruptors of the glucokinase-glucokinase regulatory protein interaction: 3. Structure-activity relationships within the aryl carbinol region of the N-arylsulfonamido-N'-arylpiperazine series.
Nishimura N, Norman MH, Liu L, Yang KC, Ashton KS, Bartberger MD, Chmait S, Chen J, Cupples R, Fotsch C, Helmering J, Jordan SR, Kunz RK, Pennington LD, Poon SF, Siegmund A, Sivits G, Lloyd DJ, Hale C, St Jean DJ.
J Med Chem (2014) 57 : 3094 -3116
PubMed 24611879 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 7.12 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3114185 1 8.40
CHEMBL3237987 1 8.30
CHEMBL3237986 1 8.22
CHEMBL3237981 1 8.22
CHEMBL3114187 1 8.05
CHEMBL3238310 1 8.00
CHEMBL3238309 1 8.00
CHEMBL3238313 1 7.89
CHEMBL3238308 1 7.89
CHEMBL3238307 1 7.89
CHEMBL3237985 1 7.80
CHEMBL3238303 1 7.77
CHEMBL3238306 1 7.75
CHEMBL3238312 1 7.62
CHEMBL3237980 1 7.55
CHEMBL3237984 1 7.55
CHEMBL3237993 1 7.46
CHEMBL3238301 1 7.35
CHEMBL3237983 1 7.33
CHEMBL3238316 1 7.31
CHEMBL3237992 1 7.21
CHEMBL3238311 1 7.17
CHEMBL3238314 1 7.16
CHEMBL3238305 1 7.16
CHEMBL3238315 1 7.09
CHEMBL3237994 1 7.00
CHEMBL3238302 1 6.74
CHEMBL3238296 1 6.63
CHEMBL3238300 1 6.59
CHEMBL3238304 1 6.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3114185 IC50 = 4.0 nM 8.40
CHEMBL3237987 IC50 = 5.0 nM 8.30
CHEMBL3237981 IC50 = 6.0 nM 8.22
CHEMBL3237986 IC50 = 6.0 nM 8.22
CHEMBL3114187 IC50 = 9.0 nM 8.05
CHEMBL3238310 IC50 = 10.0 nM 8.00
CHEMBL3238309 IC50 = 10.0 nM 8.00
CHEMBL3238313 IC50 = 13.0 nM 7.89
CHEMBL3238308 IC50 = 13.0 nM 7.89
CHEMBL3238307 IC50 = 13.0 nM 7.89
CHEMBL3237985 IC50 = 16.0 nM 7.80
CHEMBL3238303 IC50 = 17.0 nM 7.77
CHEMBL3238306 IC50 = 18.0 nM 7.75
CHEMBL3238312 IC50 = 24.0 nM 7.62
CHEMBL3237984 IC50 = 28.0 nM 7.55
CHEMBL3237980 IC50 = 28.0 nM 7.55
CHEMBL3237993 IC50 = 35.0 nM 7.46
CHEMBL3238301 IC50 = 45.0 nM 7.35
CHEMBL3237983 IC50 = 47.0 nM 7.33
CHEMBL3238316 IC50 = 49.0 nM 7.31
CHEMBL3237992 IC50 = 61.0 nM 7.21
CHEMBL3238311 IC50 = 68.0 nM 7.17
CHEMBL3238314 IC50 = 70.0 nM 7.16
CHEMBL3238305 IC50 = 69.0 nM 7.16
CHEMBL3238315 IC50 = 81.0 nM 7.09
CHEMBL3237994 IC50 = 101.0 nM 7.00
CHEMBL3238302 IC50 = 182.0 nM 6.74
CHEMBL3238296 IC50 = 235.0 nM 6.63
CHEMBL3238300 IC50 = 256.0 nM 6.59
CHEMBL3238304 IC50 = 266.0 nM 6.58
CHEMBL3238299 IC50 = 310.0 nM 6.51
CHEMBL3238297 IC50 = 320.0 nM 6.50
CHEMBL3237982 IC50 = 374.0 nM 6.43
CHEMBL3237991 IC50 = 401.0 nM 6.40
CHEMBL3237979 IC50 = 1050.0 nM 5.98
CHEMBL3237988 IC50 = 1090.0 nM 5.96
CHEMBL3238298 IC50 = 1600.0 nM 5.80
CHEMBL3237989 IC50 = 3070.0 nM 5.51
CHEMBL3237990 IC50 = 3360.0 nM 5.47
CHEMBL3237978 IC50 = 25000.0 nM 4.60