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Assay Detail

CHEMBL3240718

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCG2 (unknown origin) transfected in HEK293 cells assessed as inhibition of mitoxantrone efflux after 30 mins by flow cytometry relative to control
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Symmetric bis-chalcones as a new type of breast cancer resistance protein inhibitors with a mechanism different from that of chromones.
Winter E, Devantier Neuenfeldt P, Chiaradia-Delatorre LD, Gauthier C, Yunes RA, Nunes RJ, Creczynski-Pasa TB, Di Pietro A.
J Med Chem (2014) 57 : 2930 -2941
PubMed 24611893 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 6.22 7.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL488910 1 7.54
CHEMBL2011288 1 7.03
CHEMBL3238330 1 6.70
CHEMBL3238323 1 6.52
CHEMBL3238325 1 6.40
CHEMBL3238340 1 6.30
CHEMBL3238320 1 6.16
CHEMBL3238335 1 6.10
CHEMBL3238336 1 6.05
CHEMBL3238333 1 5.96
CHEMBL3238343 1 5.96
CHEMBL3238338 1 5.89
CHEMBL3238331 1 5.85
CHEMBL3238346 1 5.75
CHEMBL1504035 1 5.68
CHEMBL3238334 1 5.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL488910 EC50 = 29.0 nM 7.54
CHEMBL2011288 EC50 = 94.0 nM 7.03
CHEMBL3238330 EC50 = 200.0 nM 6.70
CHEMBL3238323 EC50 = 300.0 nM 6.52
CHEMBL3238325 EC50 = 400.0 nM 6.40
CHEMBL3238340 EC50 = 500.0 nM 6.30
CHEMBL3238320 EC50 = 700.0 nM 6.16
CHEMBL3238335 EC50 = 800.0 nM 6.10
CHEMBL3238336 EC50 = 900.0 nM 6.05
CHEMBL3238333 EC50 = 1100.0 nM 5.96
CHEMBL3238343 EC50 = 1100.0 nM 5.96
CHEMBL3238338 EC50 = 1300.0 nM 5.89
CHEMBL3238331 EC50 = 1400.0 nM 5.85
CHEMBL3238346 EC50 = 1800.0 nM 5.75
CHEMBL1504035 EC50 = 2100.0 nM 5.68
CHEMBL3238334 EC50 = 2300.0 nM 5.64