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Assay Detail

CHEMBL3241596

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R mutant (unknown origin) after 1.5 hr by FRET-based Z-lyte assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of 2-anilinopyrimidines bearing 3-aminopropamides as potential epidermal growth factor receptor inhibitors.
Han C, Wan L, Ji H, Ding K, Huang Z, Lai Y, Peng S, Zhang Y.
Eur J Med Chem (2014) 77 : 75 -83
PubMed 24607591 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.43 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL939 GEFITINIB 4.0 1 9.70
CHEMBL1229592 1 8.32
CHEMBL3237937 1 6.58
CHEMBL3237931 1 6.37
CHEMBL3237929 1 6.09
CHEMBL3237930 1 6.09
CHEMBL3237935 1 5.62
CHEMBL3237932 1 5.34
CHEMBL3237936 1 5.23
CHEMBL3237934 1 5.00
CHEMBL3237933 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL939 GEFITINIB IC50 = 0.2 nM 9.70
CHEMBL1229592 IC50 = 4.8 nM 8.32
CHEMBL3237937 IC50 = 262.0 nM 6.58
CHEMBL3237931 IC50 = 427.0 nM 6.37
CHEMBL3237929 IC50 = 804.0 nM 6.09
CHEMBL3237930 IC50 = 818.0 nM 6.09
CHEMBL3237935 IC50 = 2385.0 nM 5.62
CHEMBL3237932 IC50 = 4527.0 nM 5.34
CHEMBL3237936 IC50 = 5866.0 nM 5.23
CHEMBL3237934 IC50 = 10000.0 nM 5.00
CHEMBL3237933 IC50 > 10000.0 nM -