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Assay Detail

CHEMBL3243059

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to GST-thrombin-tagged human MDM2 (1 to 188) expressed in Escherichia coli assessed as inhibition of interaction with p53 in buffer containing 15% human serum incubated for 20 mins prior to p53 addition measured after 60 mins by HTRF assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

E3 ubiquitin-protein ligase Mdm2 (CHEMBL5023)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective and potent morpholinone inhibitors of the MDM2-p53 protein-protein interaction.
Gonzalez AZ, Eksterowicz J, Bartberger MD, Beck HP, Canon J, Chen A, Chow D, Duquette J, Fox BM, Fu J, Huang X, Houze JB, Jin L, Li Y, Li Z, Ling Y, Lo MC, Long AM, McGee LR, McIntosh J, McMinn DL, Oliner JD, Osgood T, Rew Y, Saiki AY, Shaffer P, Wortman S, Yakowec P, Yan X, Ye Q, Yu D, Zhao X, Zhou J, Olson SH, Medina JC, Sun D.
J Med Chem (2014) 57 : 2472 -2488
PubMed 24548297 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 8.31 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3233141 1 9.30
CHEMBL3125537 1 8.96
CHEMBL3233140 1 8.74
CHEMBL3233125 1 8.70
CHEMBL3233130 1 8.70
CHEMBL3233137 1 8.68
CHEMBL3233123 1 8.64
CHEMBL3233126 1 8.62
CHEMBL3233138 1 8.60
CHEMBL3233122 1 8.43
CHEMBL3233127 1 8.40
CHEMBL3233139 1 8.29
CHEMBL3233124 1 8.22
CHEMBL3233136 1 8.16
CHEMBL3233134 1 8.00
CHEMBL3233129 1 8.00
CHEMBL3233121 1 7.96
CHEMBL3233133 1 7.77
CHEMBL3233131 1 7.75
CHEMBL3233132 1 7.70
CHEMBL3233135 1 7.64
CHEMBL3233128 1 7.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3233141 IC50 = 0.5 nM 9.30
CHEMBL3125537 IC50 = 1.1 nM 8.96
CHEMBL3233140 IC50 = 1.8 nM 8.74
CHEMBL3233125 IC50 = 2.0 nM 8.70
CHEMBL3233130 IC50 = 2.0 nM 8.70
CHEMBL3233137 IC50 = 2.1 nM 8.68
CHEMBL3233123 IC50 = 2.3 nM 8.64
CHEMBL3233126 IC50 = 2.4 nM 8.62
CHEMBL3233138 IC50 = 2.5 nM 8.60
CHEMBL3233122 IC50 = 3.7 nM 8.43
CHEMBL3233127 IC50 = 4.0 nM 8.40
CHEMBL3233139 IC50 = 5.1 nM 8.29
CHEMBL3233124 IC50 = 6.0 nM 8.22
CHEMBL3233136 IC50 = 6.9 nM 8.16
CHEMBL3233134 IC50 = 10.0 nM 8.00
CHEMBL3233129 IC50 = 10.1 nM 8.00
CHEMBL3233121 IC50 = 11.0 nM 7.96
CHEMBL3233133 IC50 = 17.0 nM 7.77
CHEMBL3233131 IC50 = 18.0 nM 7.75
CHEMBL3233132 IC50 = 20.0 nM 7.70
CHEMBL3233135 IC50 = 23.0 nM 7.64
CHEMBL3233128 IC50 = 26.0 nM 7.58