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Assay Detail

CHEMBL3243099

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of sEH (unknown origin) assessed as substrate PHOME hydrolysis after 1 hr by fluorescence method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bifunctional epoxide hydrolase 2 (CHEMBL2409)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Anti-inflammatory components of Euphorbia humifusa Willd.
Luyen BT, Tai BH, Thao NP, Eun KJ, Cha JY, Xin MJ, Lee YM, Kim YH.
Bioorg Med Chem Lett (2014) 24 : 1895 -1900
PubMed 24679441 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 4.82 7.99

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL215387 1 7.99
CHEMBL1221722 1 4.76
CHEMBL459260 1 4.74
CHEMBL3236511 1 4.73
CHEMBL6246 ELLAGIC ACID 2.0 1 4.68
CHEMBL251254 HYPEROSIDE 1 4.66
CHEMBL3236510 1 4.62
CHEMBL226335 RUTIN 3.0 1 4.61
CHEMBL444191 1 4.56
CHEMBL250450 ISOQUERCETIN 2.0 1 4.55
CHEMBL50 QUERCETIN 3.0 1 4.47
CHEMBL3236509 1 4.44
CHEMBL245067 PHLORIZIN 1 4.38
CHEMBL3236512 1 4.33
CHEMBL233930 ASTRAGALIN 1 -
CHEMBL3236513 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL215387 IC50 = 10.3 nM 7.99
CHEMBL1221722 IC50 = 17230.0 nM 4.76
CHEMBL459260 IC50 = 18050.0 nM 4.74
CHEMBL3236511 IC50 = 18640.0 nM 4.73
CHEMBL6246 ELLAGIC ACID IC50 = 20700.0 nM 4.68
CHEMBL251254 HYPEROSIDE IC50 = 21760.0 nM 4.66
CHEMBL3236510 IC50 = 23810.0 nM 4.62
CHEMBL226335 RUTIN IC50 = 24300.0 nM 4.61
CHEMBL444191 IC50 = 27430.0 nM 4.56
CHEMBL250450 ISOQUERCETIN IC50 = 28430.0 nM 4.55
CHEMBL50 QUERCETIN IC50 = 33540.0 nM 4.47
CHEMBL3236509 IC50 = 36140.0 nM 4.44
CHEMBL245067 PHLORIZIN IC50 = 41720.0 nM 4.38
CHEMBL3236512 IC50 = 47360.0 nM 4.33
CHEMBL233930 ASTRAGALIN IC50 > 50000.0 nM -
CHEMBL3236513 IC50 > 50000.0 nM -