Assay Detail
Binding
CHEMBL3258749
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Competitive inhibition of peptidyl transferase activity of Escherichia coli ribosome using Ac[14C]-L-Phe-tRNA/puromycin as donor/acceptor substrate assessed as acetyl[14C]phenylalanylpuromycin formation by double reciprocal plot analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Subcellular | Ribosome |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Effect of sparsomycin analogues on the puromycin-peptidyl transferase reaction on ribosomes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 6 | 5.91 | 6.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2303630 | SPARSOMYCIN | 2.0 | 1 | 6.30 |
| CHEMBL115026 | — | — | 1 | 5.96 |
| CHEMBL3248507 | — | — | 1 | 5.96 |
| CHEMBL3248509 | — | — | 1 | 5.92 |
| CHEMBL3248506 | — | — | 1 | 5.70 |
| CHEMBL3248511 | — | — | 1 | 5.64 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2303630 | SPARSOMYCIN | Ki | = | 500.0 | nM | 6.30 |
| CHEMBL115026 | — | Ki | = | 1090.0 | nM | 5.96 |
| CHEMBL3248507 | — | Ki | = | 1100.0 | nM | 5.96 |
| CHEMBL3248509 | — | Ki | = | 1210.0 | nM | 5.92 |
| CHEMBL3248506 | — | Ki | = | 2000.0 | nM | 5.70 |
| CHEMBL3248511 | — | Ki | = | 2300.0 | nM | 5.64 |