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Assay Detail

CHEMBL3266757

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of purified mouse iNOS assessed as inhibition of nitric oxide-mediated oxidation of hemoglobin-A by hemoglobin capture assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL3464)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Nitric oxide synthase inhibitors that interact with both heme propionate and tetrahydrobiopterin show high isoform selectivity.
Kang S, Tang W, Li H, Chreifi G, Martásek P, Roman LJ, Poulos TL, Silverman RB.
J Med Chem (2014) 57 : 4382 -4396
PubMed 24758147 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 5.17 5.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3262022 1 5.72
CHEMBL3262024 1 5.55
CHEMBL3262025 1 5.36
CHEMBL3262019 1 5.22
CHEMBL3262026 1 5.18
CHEMBL3262018 1 4.81
CHEMBL3262017 1 4.75
CHEMBL3262023 1 4.75
CHEMBL3262020 1 -
CHEMBL3262021 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3262022 Ki = 1894.0 nM 5.72
CHEMBL3262024 Ki = 2810.0 nM 5.55
CHEMBL3262025 Ki = 4386.0 nM 5.36
CHEMBL3262019 Ki = 5990.0 nM 5.22
CHEMBL3262026 Ki = 6629.0 nM 5.18
CHEMBL3262018 Ki = 15620.0 nM 4.81
CHEMBL3262017 Ki = 17670.0 nM 4.75
CHEMBL3262023 Ki = 17916.0 nM 4.75
CHEMBL3262020 Ki > 100000.0 nM -
CHEMBL3262021 Ki > 100000.0 nM -