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Assay Detail

CHEMBL3267829

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of human HCT116 cell growth after 96 hrs by counting kit-8 analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCT-116
Confidence 1 — Organism
Curated By Autocuration

Target

HCT-116 (CHEMBL394)
Type CELL-LINE
Organism Homo sapiens

Publication

Optimizing the Physicochemical Properties of Raf/MEK Inhibitors by Nitrogen Scanning.
Aoki T, Hyohdoh I, Furuichi N, Ozawa S, Watanabe F, Matsushita M, Sakaitani M, Morikami K, Takanashi K, Harada N, Tomii Y, Shiraki K, Furumoto K, Tabo M, Yoshinari K, Ori K, Aoki Y, Shimma N, Iikura H.
ACS Med Chem Lett (2014) 5 : 309 -314
PubMed 24900832 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.08 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3092195 1 7.77
CHEMBL3092179 1 7.66
CHEMBL3092189 1 7.62
CHEMBL3263996 1 7.50
CHEMBL3264002 AVUTOMETINIB 3.0 1 7.40
CHEMBL3264000 1 7.35
CHEMBL3263999 1 6.96
CHEMBL3263995 1 6.57
CHEMBL3264001 1 6.05
CHEMBL3263994 1 5.96
CHEMBL3263997 1 -
CHEMBL3263998 1 -
CHEMBL3264003 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3092195 IC50 = 17.0 nM 7.77
CHEMBL3092179 IC50 = 22.0 nM 7.66
CHEMBL3092189 IC50 = 24.0 nM 7.62
CHEMBL3263996 IC50 = 32.0 nM 7.50
CHEMBL3264002 AVUTOMETINIB IC50 = 40.0 nM 7.40
CHEMBL3264000 IC50 = 45.0 nM 7.35
CHEMBL3263999 IC50 = 110.0 nM 6.96
CHEMBL3263995 IC50 = 270.0 nM 6.57
CHEMBL3264001 IC50 = 900.0 nM 6.05
CHEMBL3263994 IC50 = 1100.0 nM 5.96
CHEMBL3263997 IC50 > 10000.0 nM -
CHEMBL3263998 IC50 > 10000.0 nM -
CHEMBL3264003 IC50 > 10000.0 nM -