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Assay Detail

CHEMBL3268063

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged human recombinant PDE10A assessed as substrate hydrolysis using [3H]cAMP as substrate after 30 mins by two-step radiometric assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design, synthesis and pharmacological evaluation of novel polycyclic heteroarene ethers as PDE10A inhibitors: Part I.
Das S, Harde RL, Shelke DE, Khairatkar-Joshi N, Bajpai M, Sapalya RS, Surve HV, Gudi GS, Pattem R, Behera DB, Jadhav SB, Thomas A.
Bioorg Med Chem Lett (2014) 24 : 2073 -2078
PubMed 24725435 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 7.17 9.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL562318 MARDEPODECT 2.0 1 9.43
CHEMBL562513 1 8.85
CHEMBL3262297 1 7.98
CHEMBL3262293 1 7.95
CHEMBL3262298 1 7.62
CHEMBL3262303 1 7.48
CHEMBL3262291 1 7.38
CHEMBL3262294 1 7.17
CHEMBL3262295 1 7.16
CHEMBL3262290 1 6.91
CHEMBL3262292 1 6.72
CHEMBL3262296 1 6.23
CHEMBL3262302 1 6.01
CHEMBL3262300 1 5.49
CHEMBL3262299 1 5.23
CHEMBL3262285 1 -
CHEMBL3262289 1 -
CHEMBL3262286 1 -
CHEMBL3262284 1 -
CHEMBL3262288 1 -
CHEMBL3262301 1 -
CHEMBL3262287 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL562318 MARDEPODECT IC50 = 0.37 nM 9.43
CHEMBL562513 IC50 = 1.41 nM 8.85
CHEMBL3262297 IC50 = 10.4 nM 7.98
CHEMBL3262293 IC50 = 11.2 nM 7.95
CHEMBL3262298 IC50 = 24.2 nM 7.62
CHEMBL3262303 IC50 = 33.2 nM 7.48
CHEMBL3262291 IC50 = 41.5 nM 7.38
CHEMBL3262294 IC50 = 67.4 nM 7.17
CHEMBL3262295 IC50 = 68.5 nM 7.16
CHEMBL3262290 IC50 = 123.2 nM 6.91
CHEMBL3262292 IC50 = 188.2 nM 6.72
CHEMBL3262296 IC50 = 592.1 nM 6.23
CHEMBL3262302 IC50 = 971.2 nM 6.01
CHEMBL3262300 IC50 = 3217.2 nM 5.49
CHEMBL3262299 IC50 = 5936.8 nM 5.23
CHEMBL3262285 IC50 - -
CHEMBL3262289 IC50 - -
CHEMBL3262286 IC50 - -
CHEMBL3262284 IC50 - -
CHEMBL3262288 IC50 - -
CHEMBL3262301 IC50 - -
CHEMBL3262287 IC50 - -