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Assay Detail

CHEMBL3271452

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EZH2 Y641N mutant (unknown origin)
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EZH2 (CHEMBL2189110)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and Optimization of Tetramethylpiperidinyl Benzamides as Inhibitors of EZH2.
Nasveschuk CG, Gagnon A, Garapaty-Rao S, Balasubramanian S, Campbell R, Lee C, Zhao F, Bergeron L, Cummings R, Trojer P, Audia JE, Albrecht BK, Harmange JC.
ACS Med Chem Lett (2014) 5 : 378 -383
PubMed 24900844 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 5.54 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3264788 1 6.89
CHEMBL3264787 1 6.72
CHEMBL3264786 1 6.25
CHEMBL3264783 1 5.85
CHEMBL3264795 1 5.70
CHEMBL3264784 1 5.66
CHEMBL3264794 1 5.60
CHEMBL3264785 1 5.40
CHEMBL3264792 1 5.00
CHEMBL3264793 1 5.00
CHEMBL3264789 1 4.21
CHEMBL3264791 1 4.18
CHEMBL3264790 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3264788 IC50 = 130.0 nM 6.89
CHEMBL3264787 IC50 = 190.0 nM 6.72
CHEMBL3264786 IC50 = 560.0 nM 6.25
CHEMBL3264783 IC50 = 1400.0 nM 5.85
CHEMBL3264795 IC50 = 2000.0 nM 5.70
CHEMBL3264784 IC50 = 2200.0 nM 5.66
CHEMBL3264794 IC50 = 2500.0 nM 5.60
CHEMBL3264785 IC50 = 4000.0 nM 5.40
CHEMBL3264792 IC50 = 10000.0 nM 5.00
CHEMBL3264793 IC50 = 10000.0 nM 5.00
CHEMBL3264789 IC50 = 62000.0 nM 4.21
CHEMBL3264791 IC50 = 66000.0 nM 4.18
CHEMBL3264790 IC50 > 80000.0 nM -