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Assay Detail

CHEMBL3284707

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of amethopterin-resistant Lactobacillus casei thymidylate synthetase-catalyzed 5-BrdUMP dehalogenation by absorbance analysis in absence of CH2-H4-folate
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lacticaseibacillus casei
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Thymidylate synthase (CHEMBL5328)
Type SINGLE PROTEIN
Organism Lactobacillus casei

Publication

Inhibition of Lactobacillus casei thymidylate synthetase by 5-substituted 2'-deoxyuridylates. Preliminary quantitative structure-activity relationship.
Wataya Y, Santi DV, Hansch C.
J Med Chem (1977) 20 : 1469 -1473
PubMed 410930 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 5.20 5.65

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL211312 DEOXYURIDINE MONOPHOSPHATE 1 5.65
CHEMBL3274191 1 5.41
CHEMBL394429 THYMIDINE MONOPHOSPHATE 1 5.06
CHEMBL1236538 1 5.03
CHEMBL886 5-FLUORODEOXYURIDYLATE 1 4.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL211312 DEOXYURIDINE MONOPHOSPHATE Ki = 2260.0 nM 5.65
CHEMBL3274191 Ki = 3900.0 nM 5.41
CHEMBL394429 THYMIDINE MONOPHOSPHATE Ki = 8690.0 nM 5.06
CHEMBL1236538 Ki = 9390.0 nM 5.03
CHEMBL886 5-FLUORODEOXYURIDYLATE Ki = 14900.0 nM 4.83