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Assay Detail

CHEMBL3291706

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production at after 18 hrs by Griess method relative to control
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis and structure-activity relationships of guaiane-type sesquiterpene lactone derivatives with respect to inhibiting NO production in lipopolysaccharide-induced RAW 264.7 macrophages.
Chen H, Chen BY, Liu CT, Zhao Z, Shao WH, Yuan H, Bi KJ, Liu JY, Sun QY, Zhang WD.
Eur J Med Chem (2014) 83 : 307 -316
PubMed 24974350 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 4.77 5.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3289855 1 5.16
CHEMBL225304 PIMAGEDINE 2.0 1 5.06
CHEMBL3289622 1 4.83
CHEMBL3289848 1 4.76
CHEMBL3289640 1 4.74
CHEMBL3289639 1 4.65
CHEMBL3289856 1 4.59
CHEMBL3289633 1 4.57
CHEMBL3289632 1 4.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3289855 IC50 = 6980.0 nM 5.16
CHEMBL225304 PIMAGEDINE IC50 = 8680.0 nM 5.06
CHEMBL3289622 IC50 = 14800.0 nM 4.83
CHEMBL3289848 IC50 = 17410.0 nM 4.76
CHEMBL3289640 IC50 = 18330.0 nM 4.74
CHEMBL3289639 IC50 = 22340.0 nM 4.65
CHEMBL3289856 IC50 = 25720.0 nM 4.59
CHEMBL3289633 IC50 = 27200.0 nM 4.57
CHEMBL3289632 IC50 = 28960.0 nM 4.54