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Assay Detail

CHEMBL3294262

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant HIV-1 integrase strand transfer activity using 32P 5' end-labeled linear 21'mer as substrate preincubated for 30 mins prior to substrate challenge by phosphorimaging analysis
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 integrase (CHEMBL3471)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Design and discovery of flavonoid-based HIV-1 integrase inhibitors targeting both the active site and the interaction with LEDGF/p75.
Li BW, Zhang FH, Serrao E, Chen H, Sanchez TW, Yang LM, Neamati N, Zheng YT, Wang H, Long YQ.
Bioorg Med Chem (2014) 22 : 3146 -3158
PubMed 24794743 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 4.90 6.11

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8260 BAICALEIN 2.0 1 6.11
CHEMBL3288712 1 5.40
CHEMBL3288714 1 5.16
CHEMBL3288958 1 5.12
CHEMBL50 QUERCETIN 3.0 1 5.10
CHEMBL151 LUTEOLIN 2.0 1 5.05
CHEMBL3288946 1 4.46
CHEMBL3288950 1 4.30
CHEMBL3288955 1 4.16
CHEMBL3288954 1 4.10
CHEMBL3288713 1 -
CHEMBL3288943 1 -
CHEMBL3288944 1 -
CHEMBL3288945 1 -
CHEMBL28 APIGENIN 1 -
CHEMBL3288947 1 -
CHEMBL3288948 1 -
CHEMBL117 CHRYSIN 1 -
CHEMBL3288949 1 -
CHEMBL3288951 1 -
CHEMBL9352 NARINGENIN 1 -
CHEMBL3288959 1 -
CHEMBL3286423 1 -
CHEMBL3288957 1 -
CHEMBL3288956 1 -
CHEMBL44 GENISTEIN 2.0 1 -
CHEMBL3288953 1 -
CHEMBL3288960 1 -
CHEMBL3288952 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8260 BAICALEIN IC50 = 780.0 nM 6.11
CHEMBL3288712 IC50 = 4000.0 nM 5.40
CHEMBL3288714 IC50 = 7000.0 nM 5.16
CHEMBL3288958 IC50 = 7600.0 nM 5.12
CHEMBL50 QUERCETIN IC50 = 8000.0 nM 5.10
CHEMBL151 LUTEOLIN IC50 = 8900.0 nM 5.05
CHEMBL3288946 IC50 = 35000.0 nM 4.46
CHEMBL3288950 IC50 = 50000.0 nM 4.30
CHEMBL3288955 IC50 = 70000.0 nM 4.16
CHEMBL3288954 IC50 = 80000.0 nM 4.10
CHEMBL3288713 IC50 > 20000.0 nM -
CHEMBL3288943 IC50 > 100000.0 nM -
CHEMBL3288944 IC50 > 20000.0 nM -
CHEMBL3288945 IC50 > 20000.0 nM -
CHEMBL28 APIGENIN IC50 > 20000.0 nM -
CHEMBL3288947 IC50 > 20000.0 nM -
CHEMBL3288948 IC50 > 20000.0 nM -
CHEMBL117 CHRYSIN IC50 > 20000.0 nM -
CHEMBL3288949 IC50 > 20000.0 nM -
CHEMBL3288951 IC50 > 20000.0 nM -
CHEMBL9352 NARINGENIN IC50 > 20000.0 nM -
CHEMBL3288959 IC50 > 20000.0 nM -
CHEMBL3286423 IC50 > 20000.0 nM -
CHEMBL3288957 IC50 > 20000.0 nM -
CHEMBL3288956 IC50 > 20000.0 nM -
CHEMBL44 GENISTEIN IC50 > 20000.0 nM -
CHEMBL3288953 IC50 > 100000.0 nM -
CHEMBL3288960 IC50 > 20000.0 nM -
CHEMBL3288952 IC50 > 100000.0 nM -