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Assay Detail

CHEMBL3294266

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of His6-tagged HIV-1 integrase assessed as decrease in integrase-Flag-LEDGF/p75 interaction preincubated with enzyme for 30 mins followed by addition of Flag-LEDGF/p75 for 1 hr by AlphaScreen assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 integrase (CHEMBL3471)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Design and discovery of flavonoid-based HIV-1 integrase inhibitors targeting both the active site and the interaction with LEDGF/p75.
Li BW, Zhang FH, Serrao E, Chen H, Sanchez TW, Yang LM, Neamati N, Zheng YT, Wang H, Long YQ.
Bioorg Med Chem (2014) 22 : 3146 -3158
PubMed 24794743 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 5.92 6.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3286423 1 6.42
CHEMBL151 LUTEOLIN 2.0 1 6.35
CHEMBL50 QUERCETIN 3.0 1 6.22
CHEMBL3288712 1 6.03
CHEMBL3288713 1 6.01
CHEMBL44 GENISTEIN 2.0 1 5.96
CHEMBL3288960 1 5.85
CHEMBL117 CHRYSIN 1 5.85
CHEMBL28 APIGENIN 1 5.82
CHEMBL3288945 1 5.80
CHEMBL3288948 1 5.77
CHEMBL3288956 1 5.77
CHEMBL9352 NARINGENIN 1 5.77
CHEMBL3288958 1 5.75
CHEMBL3288959 1 5.75
CHEMBL8260 BAICALEIN 2.0 1 5.57
CHEMBL3288951 1 -
CHEMBL3288950 1 -
CHEMBL3288952 1 -
CHEMBL3288953 1 -
CHEMBL3288943 1 -
CHEMBL3288954 1 -
CHEMBL3288955 1 -
CHEMBL3288949 1 -
CHEMBL3288957 1 -
CHEMBL3288714 1 -
CHEMBL3288944 1 -
CHEMBL3288947 1 -
CHEMBL3288946 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3286423 IC50 = 380.0 nM 6.42
CHEMBL151 LUTEOLIN IC50 = 450.0 nM 6.35
CHEMBL50 QUERCETIN IC50 = 600.0 nM 6.22
CHEMBL3288712 IC50 = 940.0 nM 6.03
CHEMBL3288713 IC50 = 970.0 nM 6.01
CHEMBL44 GENISTEIN IC50 = 1100.0 nM 5.96
CHEMBL3288960 IC50 = 1400.0 nM 5.85
CHEMBL117 CHRYSIN IC50 = 1400.0 nM 5.85
CHEMBL28 APIGENIN IC50 = 1500.0 nM 5.82
CHEMBL3288945 IC50 = 1600.0 nM 5.80
CHEMBL3288948 IC50 = 1700.0 nM 5.77
CHEMBL3288956 IC50 = 1700.0 nM 5.77
CHEMBL9352 NARINGENIN IC50 = 1700.0 nM 5.77
CHEMBL3288958 IC50 = 1800.0 nM 5.75
CHEMBL3288959 IC50 = 1800.0 nM 5.75
CHEMBL8260 BAICALEIN IC50 = 2700.0 nM 5.57
CHEMBL3288951 IC50 > 20000.0 nM -
CHEMBL3288950 IC50 > 20000.0 nM -
CHEMBL3288952 IC50 - -
CHEMBL3288953 IC50 > 20000.0 nM -
CHEMBL3288943 IC50 > 20000.0 nM -
CHEMBL3288954 IC50 > 20000.0 nM -
CHEMBL3288955 IC50 > 20000.0 nM -
CHEMBL3288949 IC50 > 20000.0 nM -
CHEMBL3288957 IC50 > 20000.0 nM -
CHEMBL3288714 IC50 > 20000.0 nM -
CHEMBL3288944 IC50 - -
CHEMBL3288947 IC50 > 20000.0 nM -
CHEMBL3288946 IC50 > 20000.0 nM -