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Assay Detail

CHEMBL3294688

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse GAT4 expressed in HEK293 cells assessed as decrease in [3H]GABA uptake
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

GABA transporter 4 (CHEMBL3699)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis, biological evaluation and structure-activity relationship of new GABA uptake inhibitors, derivatives of 4-aminobutanamides.
Kowalczyk P, Sałat K, Höfner GC, Mucha M, Rapacz A, Podkowa A, Filipek B, Wanner KT, Kulig K.
Eur J Med Chem (2014) 83 : 256 -273
PubMed 24973660 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 4.87 5.71

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL75035 1 5.71
CHEMBL3289588 1 5.13
CHEMBL3289573 1 5.08
CHEMBL3289587 1 5.07
CHEMBL3289575 1 5.04
CHEMBL3289578 1 5.01
CHEMBL3289586 1 4.99
CHEMBL3289296 1 4.99
CHEMBL3289585 1 4.99
CHEMBL3289580 1 4.96
CHEMBL3289570 1 4.94
CHEMBL3289583 1 4.94
CHEMBL3289566 1 4.93
CHEMBL3289579 1 4.92
CHEMBL3289292 1 4.92
CHEMBL3289581 1 4.92
CHEMBL3289576 1 4.90
CHEMBL3289577 1 4.90
CHEMBL3289582 1 4.87
CHEMBL3289572 1 4.87
CHEMBL3289571 1 4.84
CHEMBL3289291 1 4.82
CHEMBL3289568 1 4.82
CHEMBL3289574 1 4.80
CHEMBL3289584 1 4.70
CHEMBL3289294 1 4.63
CHEMBL3289569 1 4.59
CHEMBL3289295 1 4.59
CHEMBL3289567 1 4.50
CHEMBL3289293 1 4.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL75035 IC50 = 1949.84 nM 5.71
CHEMBL3289588 IC50 = 7413.1 nM 5.13
CHEMBL3289573 IC50 = 8317.64 nM 5.08
CHEMBL3289587 IC50 = 8511.38 nM 5.07
CHEMBL3289575 IC50 = 9120.11 nM 5.04
CHEMBL3289578 IC50 = 9772.37 nM 5.01
CHEMBL3289586 IC50 = 10232.93 nM 4.99
CHEMBL3289296 IC50 = 10232.93 nM 4.99
CHEMBL3289585 IC50 = 10232.93 nM 4.99
CHEMBL3289580 IC50 = 10964.78 nM 4.96
CHEMBL3289570 IC50 = 11481.54 nM 4.94
CHEMBL3289583 IC50 = 11481.54 nM 4.94
CHEMBL3289566 IC50 = 11748.98 nM 4.93
CHEMBL3289579 IC50 = 12022.64 nM 4.92
CHEMBL3289581 IC50 = 12022.64 nM 4.92
CHEMBL3289292 IC50 = 12022.64 nM 4.92
CHEMBL3289576 IC50 = 12589.25 nM 4.90
CHEMBL3289577 IC50 = 12589.25 nM 4.90
CHEMBL3289582 IC50 = 13489.63 nM 4.87
CHEMBL3289572 IC50 = 13489.63 nM 4.87
CHEMBL3289571 IC50 = 14454.4 nM 4.84
CHEMBL3289568 IC50 = 15135.61 nM 4.82
CHEMBL3289291 IC50 = 15135.61 nM 4.82
CHEMBL3289574 IC50 = 15848.93 nM 4.80
CHEMBL3289584 IC50 = 19952.62 nM 4.70
CHEMBL3289294 IC50 = 23442.29 nM 4.63
CHEMBL3289569 IC50 = 25703.96 nM 4.59
CHEMBL3289295 IC50 = 25703.96 nM 4.59
CHEMBL3289567 IC50 = 31622.78 nM 4.50
CHEMBL3289293 IC50 = 37153.52 nM 4.43
CHEMBL3289290 IC50 = 58884.37 nM 4.23