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Assay Detail

CHEMBL3363226

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human GAT1 transfected in CHO cells assessed as [3H]GABA uptake after 20 mins by liquid scintillation counting analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent GABA transporter 1 (CHEMBL1903)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Conformationally Restricted GABA with Bicyclo[3.1.0]hexane Backbone as the First Highly Selective BGT-1 Inhibitor.
Kobayashi T, Suemasa A, Igawa A, Ide S, Fukuda H, Abe H, Arisawa M, Minami M, Shuto S.
ACS Med Chem Lett (2014) 5 : 889 -893
PubMed 25147609 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.94 4.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1446457 1 4.94
CHEMBL75035 1 -
CHEMBL3326391 1 -
CHEMBL3040189 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1446457 IC50 = 11400.0 nM 4.94
CHEMBL75035 IC50 > 100000.0 nM -
CHEMBL3326391 IC50 > 100000.0 nM -
CHEMBL3040189 IC50 > 100000.0 nM -